Novel method to leukoreduce murine blood for transfusion: how to reduce animal usage. Issue 1 (29th September 2015)

• Record Type: Journal Article
• Authors: Fischer, Dania; Büssow, Julian; Meybohm, Patrick; Zacharowski, Kai; Jennewein, Carla
• Is Part Of: Transfusion. Volume 56:Issue 1(2016:Feb.)
• Page Start: 146
• Page End: 152
• Publication Date: 2015-09-29
• Subjects: Hematology -- Periodicals; Blood -- Transfusion -- Periodicals; Blood Group Antigens -- Periodicals; Blood Preservation -- Periodicals; Blood Transfusion -- Periodicals; 615
• DOI: 10.1111/trf.13335
• Languages: English
• ISSNs: 0041-1132

Abstract

Abstract : BACKGROUND: Basic research on the pathomechanisms of transfusion‐related adverse events depends on murine transfusion models, in which leukoreduction (LR) is a prevalent standard. The commonly used neonatal LR filter (LRF) is associated with considerable animal numbers. A more efficient method would help support the guiding principles of "replacement, reduction, refinement" (3Rs). STUDY DESIGN AND METHODS: Blood from C57BL/6 and C57BL/6‐Tg(UBC‐GFP)30Scha/J mice was leukoreduced using 1) a neonatal LRF, 2) a syringe LRF, or 3) CD45 microbeads. Product quality was assessed according to US Food and Drug Administration (FDA) standards. White blood cell numbers were analyzed by flow cytometry; hemoglobin concentrations and hematocrit were measured and in vivo posttransfusion recoveries were determined after 2 weeks of storage. RESULTS: Using the neonatal filter, a LR of 99.56% was achieved with wastage of 12.4 mL in comparison to 99.68% and 1‐mL hold‐up volume with the syringe filter and 99.11 ± 0.24% LR and 0.1‐mL wastage using microbeads. All techniques achieved FDA quality standards, apart from posttransfusion recovery rate, which was only reached by the microbeads‐based technique. CONCLUSION: LR with CD45 microbeads not only reduces animal usage but also provides a more efficacious method regarding posttransfusion red blood cell recovery and, hence, provides a promising alternative to commonly used methods.