Variation in fluxes estimated from nitrogen isotope discrimination corresponds with independent measures of nitrogen flux in Populus balsamifera L. (19th October 2015)

• Record Type: Journal Article
• Authors: Kalcsits, Lee A.; Guy, Robert D.
• Is Part Of: Plant, cell and environment. Volume 39:Number 2(2016)
• Page Start: 310
• Page End: 319
• Publication Date: 2015-10-19
• Subjects: isotope discrimination -- flux analysis -- nitrogen; Plant physiology -- Periodicals; Plant cells and tissues -- Periodicals; Plant communities -- Periodicals; 581.105
• DOI: 10.1111/pce.12614
• Languages: English
• ISSNs: 0140-7791

Abstract

Abstract: Acquisition of mineral nitrogen by roots from the surrounding environment is often not completely efficient, in which a variable amount of leakage (efflux) relative to gross uptake (influx) occurs. The efflux/influx ratio ( E / I ) is, therefore, inversely related to the efficiency of nutrient uptake at the root level. Time‐integrated estimates of E / I and other nitrogen‐use traits may be obtainable from variation in stable isotope ratios or through compartmental analysis of tracer efflux (CATE) using radioactive or stable isotopes. To compare these two methods, Populus balsamifera L. genotypes were selected, a priori, for high or low nitrogen isotope discrimination. Vegetative cuttings were grown hydroponically, and E / I was calculated using an isotope mass balance model (IMB) and compared to E / I calculated using 15 N CATE. Both methods indicated that plants grown with ammonium had greater E / I than nitrate‐grown plants. Genotypes with high or low E/I using CATE also had similarly high or low estimates of E/I using IMB, respectively. Genotype‐specific means were linearly correlated (r = 0.77; P = 0.0065). Discrepancies in E / I between methods may reflect uncertainties in discrimination factors for the assimilatory enzymes, or temporal differences in uptake patterns. By utilizing genotypes with known variation in nitrogen isotope discrimination, a relationship between nitrogen isotope discrimination and bidirectional nitrogen fluxes at the root level was observed. Abstract : In plants, nitrogen uptake is not unidirectional and efflux regularly occurs in conjunction with influx. Nitrogen isotope discrimination in an isotope mass balance model has the potential to be an effective phenotyping approach for this trait in woody plants. Here, we report that estimates of nitrogen fluxes by the isotope mass balance model for Populus balsamifera grown under steady‐state conditions correspond to independent measures of nitrogen flux measured by compartmental analysis of tracer efflux. This research contributes to the further development of a nitrogen isotope discrimination model that better describes complex, dynamic nitrogen fluxes in plants.