Manipulation of bacteriophages with dielectrophoresis on carbon nanofiber nanoelectrode arrays. Issue 7 (2nd April 2013)

• Record Type: Journal Article
• Authors: Madiyar, Foram R.; Syed, Lateef U.; Culbertson, Christopher T.; Li, Jun
• Is Part Of: Electrophoresis. Volume 34:Issue 7(2013:Apr.)
• Page Start: NA
• Page End: NA
• Publication Date: 2013-04-02
• Subjects: Bacteriophage -- Dielectrophoresis -- Nanoelectrode array -- Vertically aligned carbon nanofibers -- Virus detection; Electrophoresis -- Periodicals; Electrophoresis -- Periodicals; 541.372
• DOI: 10.1002/elps.201370071
• Languages: English
• ISSNs: 0173-0835

Abstract

Abstract : Electrophoresis 2013, 34, 1123𠄃1130. DOI: 10.1002/elps.201200486 A nanoelectrode array dielectrophoresis chip made of vertically aligned carbon nanofibers versus a macroscale indium tin oxide covered glass slide was integrated in a microfluidic device in a "points‐and‐lid" configuration. Upon applying an AC voltage of 10 Vpp at 10 kHz, a high electric field gradient was generated at the carbon nanofiber tips, which were able to capture over 60% of virus particles (bacteriophages) in a dilute (∼9 × 10 4 pfu mL ‐1 ) fluidic flow by dielectrophoresis. At high concentrations, the virus particles were highly polarized to form a chain‐like structure showing interesting "lighting" patterns.