Desorption electrospray ionization‐based imaging of interaction between vascular graft and human body. Issue 1 (25th February 2015)

• Record Type: Journal Article
• Authors: Bodzon‐Kulakowska, Anna; Drabik, Anna; Mystkowska, Joanna; Chlabicz, Michal; Gacko, Marek; Dabrowski, Jan R.; Mielczarek, Przemyslaw; Silberring, Jerzy; Suder, Piotr
• Is Part Of: Journal of biomedical materials research. Volume 104:Issue 1(2016:Jan.)
• Page Start: 192
• Page End: 196
• Publication Date: 2015-02-25
• Subjects: molecular imaging -- vascular graft -- cholesterol plaque; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1002/jbm.b.33385
• Languages: English
• ISSNs: 1552-4973

Abstract

Abstract: The desorption electrospray ionization‐mass spectrometry imaging (DESI‐MSI) is known as a fast and convenient MS‐based method for lipid imaging in various biological materials. Here, we applied this technique to visualize lipid distribution in a vascular graft removed from a patient's body. This is a good example of the DESI system capabilities toward imaging of interaction between artificial material and living tissues. Detailed analysis allowed for visualization of the spatial distribution of selected lipids in this implanted, artificial material. Not only DESI‐MSI allowed visualization of lipid distribution in the investigated material but also enabled identification of the detected molecular species using MS/MS. Here, this technique was successfully used to evaluate the saturation and spatial distribution of endogenous lipids in the artificial vascular graft. Unambiguous identification of the lipids was done with the aid of fragmentation procedure. We also showed that various lipids localize preferably in graft material or internal plaque existing inside the graft. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 192–196, 2016.