Rapid SERS monitoring of lipid‐peroxidation‐derived protein modifications in cells using photonic crystal fiber sensor. Issue 1 (14th September 2015)

• Record Type: Journal Article
• Authors: Gong, Tianxun; Zhang, Nan; Kong, Kien Voon; Goh, Douglas; Ying, Cui; Auguste, Jean‐Louis; Shum, Perry Ping; Wei, Lei; Humbert, Georges; Yong, Ken‐Tye; Olivo, Malini
• Is Part Of: Journal of biophotonics. Volume 9:Issue 1/2(2016)
• Page Start: 32
• Page End: 37
• Publication Date: 2015-09-14
• Subjects: side channel PCF -- linoleamide alkyne -- lipid peroxidation -- HepG2 cell -- Surface enhanced Raman spectroscopy -- gold nanoparticles; Photonics -- Periodicals; Optical materials -- Periodicals; Optics -- Periodicals; Medical instruments and apparatus -- Periodicals; 621.3605
• DOI: 10.1002/jbio.201500168
• Languages: English
• ISSNs: 1864-063X

Abstract

Abstract : We proposed a side channel photonic crystal fiber (SC‐PCF) based Surface enhanced Raman spectroscopy (SERS) platform which is able to accurately monitor lipid peroxidation derived protein modifications in cells. This platform incorporates linoleamide alkyne (LAA), which is oxidized and subsequently modifies proteins in cells with alkyne functional group upon lipid peroxidation. By loading the side channel of SC‐PCF with a mixture of gold nanoparticles and LAA treated cells, and subsequently measuring the interference‐free alkyne Raman peak from these proteins in cells, strong SERS signal was obtained. The platform provides a method for the rapid monitoring of lipid peroxidation derived protein modification in cells. Abstract : Lipid peroxidation refers to the oxidative degradation of lipids which resulting in cell damage. A SERS platform is reported to detect lipid peroxidation derived protein modifications in cells using a specialty designed optical fiber. This platform incorporates linoleamide alkyne (LAA), for the detection of lipid‐peroxidation in cells. LAA is oxidized and readily modify proteins in cells upon lipid peroxidation. This process can be subsequently monitored by alkyne Raman peak at 2113 cm –1 .