An improved method for qPCR detection of three Phytophthora spp. in forest and woodland soils in northern Britain. Issue 6 (28th October 2015)

• Record Type: Journal Article
• Authors: Elliot, M.; Schlenzig, A.; Harris, C. M.; Meagher, T. R.; Green, S.
• Is Part Of: Forest pathology. Volume 45:Issue 6(2015:Dec.)
• Page Start: 537
• Page End: 539
• Publication Date: 2015-10-28
• Subjects: Trees -- Diseases and pests -- Periodicals; Trees -- Effect of air pollution on -- Periodicals; Forests and forestry -- Environmental aspects -- Periodicals; 634.9
• DOI: 10.1111/efp.12224
• Languages: English
• ISSNs: 1437-4781

Abstract

Summary: Using TaqMan qPCR assays, DNA of P. ramorum, P. kernoviae and P. austrocedri was detected in 500 g soil samples collected from twelve infected forest and woodland sites in northern Britain. Phytophthora DNA was also amplified in soil adhering to boots after walking transects along footpaths or animal trails. At two sites, Phytophthora DNA was detected in soil over a 4‐year period following removal of infected hosts. This new method enabling assessment of larger quantities of soil demonstrates the contamination risk of these pathogens in soil at infected sites and improves our understanding of the mechanisms of persistence and spread.