Determination of bisphenol A‐glucuronide in human urine using ultrahigh‐pressure liquid chromatography/tandem mass spectrometry. (10th January 2016)

• Record Type: Journal Article
• Authors: Hauck, Zane Z.; Huang, Ke; Li, Guannan; van Breemen, Richard B.
• Is Part Of: Rapid communications in mass spectrometry. Volume 30:Number 3(2016)
• Page Start: 400
• Page End: 406
• Publication Date: 2016-01-10
• Subjects: Mass spectrometry -- Periodicals; 543.65
• DOI: 10.1002/rcm.7450
• Languages: English
• ISSNs: 0951-4198

Abstract

Abstract : Rationale: Used widely as a plasticizer and as a monomer for plastics, bisphenol A (BPA) is under investigation as a possible endocrine disrupter. As an indication of systemic exposure, a fast and accurate assay was developed for the major BPA metabolite in human urine, BPA‐monoglucuronide (BPA‐G), using ultrahigh‐pressure liquid chromatography/tandem mass spectrometry (UHPLC/MS/MS). Methods: Urine samples were prepared using solid‐phase mixed‐mode reversed‐phase/anion‐exchange extraction. BPA‐G was measured using UHPLC/MS/MS with an amide UHPLC column interfaced to a triple‐quadrupole mass spectrometer equipped with negative ion electrospray, collision‐induced dissociation and selected reaction monitoring. [ 13 C12 ]‐BPA‐G was used as a surrogate standard. Results: By measuring the glucuronide metabolite of BPA, potential interference due to BPA contamination from containers, solvents, pipette, etc., was avoided. The standard curve had a linear regression coefficient of 0.999, and the intra‐ and inter‐assay variations were less than 10%. The assay was validated according to FDA guidelines. Conclusions: A fast, accurate, and highly selective method for the determination of BPA‐G in human urine was developed and validated using UHPLC/MS/MS. This method is suitable for assessing human exposure to BPA. Copyright © 2016 John Wiley & Sons, Ltd.