A pig‐to‐mouse coronary artery transplantation model for investigating the pathogenicity of anti‐pig antibody. (22nd October 2015)

• Record Type: Journal Article
• Authors: Lin, Yi; Miyagi, Naoto; Byrne, Guerard W.; Du, Zeji; Kogelberg, Heide; Gazi, Mozammel H.; Tazelaar, Henry D.; Wang, Chunsheng; McGregor, Christopher G. A.
• Is Part Of: Xenotransplantation. Volume 22:Number 6(2015:Nov./Dec.)
• Page Start: 458
• Page End: 467
• Publication Date: 2015-10-22
• Subjects: anti‐pig antibody -- coronary artery transplantation -- delayed xenograft rejection -- pig‐to‐mouse chimera -- small animal transplantation model; Xenografts -- Periodicals; Transplantation of organs, tissues, etc -- Periodicals; 617.95
• DOI: 10.1111/xen.12198
• Languages: English
• ISSNs: 0908-665X

Abstract

Abstract: Background: Rejection of Gal‐free (GTKO) donor pig cardiac xenografts is strongly associated with vascular non‐Gal antibody binding, endothelial cell (EC) injury, and activation and microvascular thrombosis. We adopted a pig‐to‐SCID/beige small animal transplant model to compare the pathogenicity of baboon and human anti‐pig antibody. Methods: Wild‐type (GT + ) or GTKO porcine coronary arteries (PCAs) were transplanted into the infrarenal aorta of SCID/beige mice. Three days after transplant, recipients were infused with anti‐pig antibody (anti‐SLA class I, an isotype control, naive or sensitized baboon serum, or naive human serum). PCAs were recovered 24 h after antibody infusion and examined using histology, immunohistochemistry, and in situ hybridization. Results: Dose‐dependent intragraft thrombosis occurred after infusion of anti‐SLA I antibody (but not isotype control) in GT + and GTKO PCA recipients. Naive baboon serum induced thrombosis in GT + grafts. Thrombosis was significantly reduced by pre‐treating naive baboon serum with Gal polymer and not observed when this serum was infused to GTKO PCA recipients. Naive human serum caused dose‐dependent intragraft thrombosis of GTKO PCAs. In all cases, thrombosis involved graft‐specific vascular antibody and complement deposition, macrophage adherence, EC delamination, and subendothelial thrombus formation. Conclusions: This study provides the first direct in vivo comparison of the pathogenicity of naive human and baboon serum. The results suggest that human preformed non‐Gal antibody may have increased pathogenicity compared to baboon. This model, which showed a rejected graft histopathology similar to antibody‐mediated rejection in cardiac xenotransplantation, may be useful to assess the pathogenicity of individual protein or carbohydrate specific non‐Gal reactive antibodies.