A Galactose Oxidase Biosensor Based on Graphene Composite Film for the Determination of Galactose and Dihydroxyacetone. Issue 1 (6th October 2015)

• Record Type: Journal Article
• Authors: Xie, Jia; Chen, Chao; Zhou, Yuanqing; Fei, Junjie; Ding, Yonglan; Zhao, Jia
• Is Part Of: Electroanalysis. Volume 28:Issue 1(2016)
• Page Start: 183
• Page End: 188
• Publication Date: 2015-10-06
• Subjects: Galactose oxidase -- Graphene -- Gold nanoparticles -- Direct electron transfer -- Biosensor; Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.201500486
• Languages: English
• ISSNs: 1040-0397

Abstract

Abstract: A novel amperometric biosensor was developed by immobilizing galactose oxidase (GAox) on poly (L‐lactide) stabilized gold nanoparticles (Au@PLLA) modified graphene nano‐composite film (RGO‐Au@PLLA/GAox). The direct electron transfer (DET) of GAox was achieved. Cyclic voltammetry of the GAox in the RGO‐Au@PLLA film revealed a pair of well‐defined and quasireversible peaks at about −0.137 V (vs. SCE), which is characterized of the Cu(II)/Cu(I) redox couple for the galactose oxidase. Amperometric response was measured as a function for the determination of galactose (GA) and dihydroxyacetone (DHA). The constructed biosensor also possesses good stability, reproducibility and anti‐interference ability. Abstract :