Performance evaluation of the Sysmex XN‐1000 hematology analyzer in assessment of the white blood cell count differential in pediatric specimens. (26th September 2015)

• Record Type: Journal Article
• Authors: Becker, P.‐H.; Fenneteau, O.; Da Costa, L.
• Is Part Of: International journal of laboratory hematology. Volume 38:Number 1(2016:Feb.)
• Page Start: 54
• Page End: 63
• Publication Date: 2015-09-26
• Subjects: Automated blood cells analyzers -- WBC differentials -- pediatrics -- Sysmex XN‐1000 -- flags; Hematology -- Periodicals; Blood -- Diseases -- Periodicals; Hematology -- Periodicals; 616.15005
• DOI: 10.1111/ijlh.12436
• Languages: English
• ISSNs: 1751-5521

Abstract

Abstract: Introduction: The automated XN‐1000 hematology analyzer enables to perform a blood cell count and a leukocyte differential. When abnormal cells were detected, a flag was generated by the analyzer and a manual microscopic examination of the corresponding blood film was performed. Methods: We compared the white blood cell differentials provided by the automated hematology analyzer XN‐1000 in a pediatric population ( n = 765) with those obtained through microscopic examination by cytologists and those obtained using a previous version of this analyzer, the XE‐2100. Leukocytes count as well as flags sensitivity and specificity was analyzed. Results: The leukocytes count provided by the analyzer is in good accordance with the differential obtained by manual count in children older than 3 months. The sensitivity for blast detection is 99% and the detection of reactive cells is 63%. The flag specificity remains low (<35%) for blood samples collected from infants between 8 days and 2 years of age, but increases up to 67% thereafter. The results obtained with the XN‐1000 analyzer show an improvement in comparison with those obtained with the XE‐2100 analyzer. Conclusion: The automated WBC differential provided by the XN‐1000 analyzer in the pediatric setting is accurate, but a meticulous microscopic examination of blood smears remains necessary for infants up to 3 months of age to validate the analyzer flags.