Characterization of the antioxidant activity of aglycone and glycosylated derivatives of hesperetin: an in vitro and in vivo study. Issue 2 (15th September 2015)

• Record Type: Journal Article
• Authors: de Souza, Verônica Trícoli; de Franco, Élida Paula Dini; de Araújo, Maria Elisa Melo Branco; Messias, Márcia Cristina Fernandes; Priviero, Fernanda Bruschi Marinho; Frankland Sawaya, Alexandra C. H.; de Oliveira Carvalho, Patricia
• Is Part Of: Journal of molecular recognition. Volume 29:Issue 2(2016)
• Page Start: 80
• Page End: 87
• Publication Date: 2015-09-15
• Subjects: hesperetin -- bioavailability -- lipid profile -- oxidative‐antioxidative system; Molecular recognition -- Periodicals; Models, Molecular -- Periodicals; Molecular Conformation -- Periodicals; Molecular Sequence Data -- Periodicals; Molecular Structure -- Periodicals; Carrier Proteins -- Periodicals; 572.8
• DOI: 10.1002/jmr.2509
• Languages: English
• ISSNs: 0952-3499

Abstract

Abstract : The flavonoids are mainly present in Citrus fruits as their glycosyl derivatives. This study was conducted comparing in vitro xanthine oxidase inhibitory activity of the aglycone hesperetin and its glycosylated forms (hesperidin and G‐hesperidin) and their effects on the plasma lipid profile and the oxidative–antioxidative system (TBARS and antioxidant enzymes) in rats. The concentrations of the major conjugated metabolites in rat plasma after oral administration of these compounds were also determined. Wistar male rats were randomly assigned to three groups (n = 6) supplemented for 30 days with 1 mmol/kg body mass of hesperetin, hesperidin or G‐hesperidin. Hesperetin was a stronger xanthine oxidase inhibitor (IC50 = 53 μM and Ki = 17.3 μM) than the glycosylate derivatives. Supplementation with the three compounds led to a lower (more favorable) atherogenic index, and an antioxidant preventive effect from the increase of hepatic superoxide dismutase was observed associated to HT supplementation, possibly because of the higher level of hesperetin‐glucuronide in rat plasma. Copyright © 2015 John Wiley & Sons, Ltd. Abstract : The influence of glycosylation of hesperitin on the kinetic parameters of XO inhibition reaction ( in vitro ), on the plasma lipid profile, on the oxidative–antioxidative system and the bioavailability of compounds in rats were evaluated. Hesperitin aglycone showed more potent in vitro XO inhibitory activity and had a similar in vivo response than the glycosylated derivatives.