Fundamentals of protein interaction network mapping. Issue 12 (17th December 2015)

• Record Type: Journal Article
• Authors: Snider, Jamie; Kotlyar, Max; Saraon, Punit; Yao, Zhong; Jurisica, Igor; Stagljar, Igor
• Is Part Of: Molecular systems biology. Volume 11:Issue 12(2015:Dec.)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2015-12-17
• Subjects: bioinformatics -- interactome mapping -- PPI technologies -- protein‐protein interactions (PPIs) -- proteomics; Molecular biology -- Periodicals; Systems biology -- Periodicals; 572.8
• DOI: 10.15252/msb.20156351
• Languages: English
• ISSNs: 1744-4292

Abstract

Abstract: Studying protein interaction networks of all proteins in an organism ("interactomes") remains one of the major challenges in modern biomedicine. Such information is crucial to understanding cellular pathways and developing effective therapies for the treatment of human diseases. Over the past two decades, diverse biochemical, genetic, and cell biological methods have been developed to map interactomes. In this review, we highlight basic principles of interactome mapping. Specifically, we discuss the strengths and weaknesses of individual assays, how to select a method appropriate for the problem being studied, and provide general guidelines for carrying out the necessary follow‐up analyses. In addition, we discuss computational methods to predict, map, and visualize interactomes, and provide a summary of some of the most important interactome resources. We hope that this review serves as both a useful overview of the field and a guide to help more scientists actively employ these powerful approaches in their research. Abstract : A practical guide to the fundamentals of protein interaction network mapping, providing information to help researchers make effective use of proteomics approaches. A range of new and well‐established experimental and computational methods and resources are covered.