Long‐term maintenance of a microfluidic 3D human liver sinusoid. Issue 1 (26th August 2015)

• Record Type: Journal Article
• Authors: Prodanov, Ljupcho; Jindal, Rohit; Bale, Shyam Sundhar; Hegde, Manjunath; McCarty, William J.; Golberg, Inna; Bhushan, Abhinav; Yarmush, Martin L.; Usta, Osman Berk
• Is Part Of: Biotechnology and bioengineering. Volume 113:Issue 1(2016)
• Page Start: 241
• Page End: 246
• Publication Date: 2015-08-26
• Subjects: liver‐on‐a‐chip -- microfludics -- liver sinusoid -- hepatocytes -- 3D microtissue; Biotechnology -- Periodicals; Bioengineering -- Periodicals; 660.6
• DOI: 10.1002/bit.25700
• Languages: English
• ISSNs: 0006-3592

Abstract

ABSTRACT: The development of long‐term human organotypic liver‐on‐a‐chip models for successful prediction of toxic response is one of the most important and urgent goals of the NIH/DARPA's initiative to replicate and replace chronic and acute drug testing in animals. For this purpose, we developed a microfluidic chip that consists of two microfluidic chambers separated by a porous membrane. The aim of this communication is to demonstrate the recapitulation of a liver sinusoid‐on‐a‐chip, using human cells only for a period of 28 days. Using a step‐by‐step method for building a 3D microtissue on‐a‐chip, we demonstrate that an organotypic in vitro model that reassembles the liver sinusoid microarchitecture can be maintained successfully for a period of 28 days. In addition, higher albumin synthesis (synthetic) and urea excretion (detoxification) were observed under flow compared to static cultures. This human liver‐on‐a‐chip should be further evaluated in drug‐related studies. Biotechnol. Bioeng. 2016;113: 241–246. © 2015 Wiley Periodicals, Inc. Abstract : A two chamber microfluidic chip is used to recapitulate liver function in vitro with human primary hepatocytes and three cells of human origin.