BRCA‐mutated Invasive Breast Carcinomas: Immunohistochemical Analysis of Insulin‐like Growth Factor II mRNA‐binding Protein (IMP3), Cytokeratin 8/18, and Cytokeratin 14. Issue 6 (22nd September 2015)

• Record Type: Journal Article
• Authors: Mohanty, Sambit K.; Lai, Jin‐Ping; Gordon, Ora K.; Pradhan, Dinesh; Bose, Shikha; Dadmanesh, Farnaz
• Is Part Of: Breast journal. Volume 21:Issue 6(2015:Nov./Dec.)
• Page Start: 596
• Page End: 603
• Publication Date: 2015-09-22
• Subjects: BRCA mutation -- CK14 -- CK8/18 -- immunohistochemistry -- IMP3 -- invasive breast carcinoma; Breast -- Diseases -- Periodicals; Breast -- Cancer -- Periodicals; 618.19
• DOI: 10.1111/tbj.12494
• Languages: English
• ISSNs: 1075-122X

Abstract

Abstract: To evaluate the expression of insulin‐like growth factor II mRNA‐binding protein (IMP3), CK8/18, and CK14 in BRCA mutated and sporadic invasive breast carcinoma. Immunohistochemistry for IMP3, CK8/18, and CK14 was performed on 39 cases of invasive breast carcinomas with BRCA mutation (24 BRCA1, 14 BRCA2, and 1 dual BRCA1/BRCA2) and 54 cases of sporadic invasive breast carcinomas. The relationship between the IMP3, CK8/18, and CK14 and the tumor grade and molecular phenotypes were analyzed. IMP3, CK8/18, and CK14 positivity were present in 20 (51%), 22 (56%), and 14 (36%) of 39 BRCA‐mutated breast carcinomas, and 11 (20%), 53 (98%), and 24 (44%) of 54 sporadic breast carcinomas respectively. The rates of IMP3 expression and absence of CK8/18 (44% versus 2%) in BRCA‐mutated breast carcinomas was significantly higher than the sporadic breast carcinomas (p = 0.002 and p < 0.001). No significant difference was observed for CK14 among the two groups (p = 0.408). No significant difference was observed among BRCA1‐related and BRCA2‐related breast carcinomas in the immunoprofile for IMP3, CK8/18, and CK14. No significant correlation was identified between the expression of IMP3 and CK8/18 and the tumor grade in both BRCA‐mutated and sporadic breast carcinomas (p > 0.05). In cases with luminal A and B phenotypes, the rates of expression of IMP3 and loss of CK8/18 were significantly higher in BRCA‐mutated as compared to sporadic breast carcinoma (p < 0.001). In cases with basal‐like phenotype, the absence of CK8/18 expression was significantly higher in BRCA‐mutated breast carcinomas (54% versus 0%, p = 0.001), while no difference was observed for IMP3 expression (p = 0.435). Regardless of mutation type, histologic grade, or molecular phenotype, the absence of CK8/18 expression and presence of IMP3 expression are seen at much higher rate in BRCA mutated breast carcinomas.