Multidimensional preparative liquid chromatography to isolate flavonoids from bergamot juice and evaluation of their anti‐inflammatory potential. Issue 24 (24th November 2015)

• Record Type: Journal Article
• Authors: Russo, Marina; Dugo, Paola; Marzocco, Stefania; Inferrera, Veronica; Mondello, Luigi
• Is Part Of: Journal of separation science. Volume 38:Issue 24(2015:Dec.)
• Page Start: 4196
• Page End: 4203
• Publication Date: 2015-11-24
• Subjects: Bergamot juice -- Flavonoids -- Macrophages -- Multidimensional chromatography -- Preparative high‐performance liquid chromatography; Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.201500878
• Languages: English
• ISSNs: 1615-9306

Abstract

Abstract : Important objectives of a high‐performance liquid chromatography preparative process are: purity of products isolated, yield, and throughput. The multidimensional preparative liquid chromatography method used in this work was developed mainly to increase the throughput; moreover purity and yield are increased thanks to the automated collection of the molecules based on the intensity of a signal generated from the mass spectrometer detector, in this way only a specific product can be targeted. This preparative system allowed, in few analyses both in the first and second dimensions, the isolation of eight pure compounds present at very different concentration in the original sample with high purity (>95%) and yield, which showed how the system is efficient and versatile. Pure molecules were used to validate the analytical method and to test the anti‐inflammatory and antiproliferative potential of flavonoids. The contemporary presence, in bergamot juice, of all the flavonoids together increases the anti‐inflammatory effect with respect to the single compound alone.