Optimization of nanofibrous silk fibroin scaffold as a delivery system for bone marrow adherent cells: in vitro and in vivo studies. (21st March 2015)

• Record Type: Journal Article
• Authors: Gholipourmalekabadi, Mazaher; Mozafari, Masoud; Bandehpour, Mojgan; Salehi, Mohammad; Sameni, Marzieh; Caicedo, Hector Hugo; Mehdipour, Ahmad; Hamidabadi, Hatef Ghasemi; Samadikuchaksaraei, Ali; Ghanbarian, Hossein
• Is Part Of: Biotechnology and applied biochemistry. Volume 62:Number 6(2015)
• Page Start: 785
• Page End: 794
• Publication Date: 2015-03-21
• Subjects: silk fibroin -- bone marrow adherent cells -- electrospinning -- nanofibrous scaffold -- tissue engineering; Biotechnology -- Periodicals; Biochemical engineering -- Periodicals; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Genetic Techniques -- Periodicals; Microbiological Techniques -- Periodicals; 660.6
• DOI: 10.1002/bab.1324
• Languages: English
• ISSNs: 0885-4513

Abstract

Abstract: Electrospun silk fibroin nanofibrous scaffolds (ESFNSs) were successfully prepared by electrospinning of various Bombyx mori silk fibroin concentrations (10, 12, and 14% in formic acid). After characterizing the purified silk fibroin, the morphology, porosity, fibers' diameter, and uniformity of the prepared scaffolds were examined in detail. In addition, biological responses such as effects on bone marrow cell viability, cytotoxicity, and cell adhesion were evaluated in vitro . Biocompatibility and bioactivity properties of the ESFNSs were evaluated in vitro and in vivo by cell culturing and subcutaneous implantation in rat models for 7 and 28 days, respectively. According to the obtained results, no beaded fibers were seen in any of the prepared scaffolds, whereas ESFNS‐10% provided more uniformity and porosity with nanoscaled fibers (90 ± 0.021 nm). Furthermore, the scaffolds also showed good cell adhesion and spreading (68.7 ± 11.8 and 7.6 ± 3.3 total length and width, respectively) with no detectable effect on cell viability and cytotoxicity. The in vivo biocompatibility evaluation indicated that the scaffolds did not stimulate detectable cellular inflammatory response (lymphocytes) and increased the total cell number (cellularity) in the implantation area. Furthermore, the results suggest the potential use of the prepared ESFNS‐10% bone marrow cell constructs in direct implantation for tissue engineering applications.