Molecular cloning and characterization of a novel carotenoid cleavage dioxygenase 1 from Lycium chinense. (30th March 2015)

• Record Type: Journal Article
• Authors: Tian, Xiaowei; Ji, Jing; Wang, Gang; Jin, Chao; Guan, Chunfeng; Wu, Guangxia
• Is Part Of: Biotechnology and applied biochemistry. Volume 62:Number 6(2015)
• Page Start: 772
• Page End: 779
• Publication Date: 2015-03-30
• Subjects: Lycium chinense -- carotenoid cleavage dioxygenase -- aroma compound -- expression; Biotechnology -- Periodicals; Biochemical engineering -- Periodicals; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Genetic Techniques -- Periodicals; Microbiological Techniques -- Periodicals; 660.6
• DOI: 10.1002/bab.1327
• Languages: English
• ISSNs: 0885-4513

Abstract

Abstract: Carotenoids are key precursor for aroma compounds in plants. Although the fruit of Lycium chinense contains numerous carotenoids, the formation mechanism of aroma compounds in L. chinense is still poorly understood. In this study, a new carotenoid cleavage dioxygenase (termed LmCCD1 ) was identified from the leaves of L. chinense . Expression analysis by semiquantitative PCR reveals that LmCCD1 gene is expressed in different tissues of L. chinense, and dominant expression of LmCCD1 gene was found in leaves, flowers, and ripe fruits. In addition, the expression level of LmCCD1 in fruits is in accordance with the content of β‐ionone. Finally, recombinantly expressed LmCCD1 can cleave β‐carotene and lycopene to produce β‐ionone and pseudoionone in in vitro assays. These results indicate that LmCCD1 a novel carotenoids cleavage dioxygenase gene that may regulate the metabolic pathways responsible for aroma metabolite production (such as β‐ionone and pseudoionone) in L. chinense has been identified.