Anti‐cancer Herbal Drug Berberine – Sensitive Determination Using Boron‐doped Diamond Electrode. Issue 12 (14th July 2015)

• Record Type: Journal Article
• Authors: Stanković, Dalibor M.; Samphao, Anchalee; Kalcher, Kurt
• Is Part Of: Electroanalysis. Volume 27:Issue 12(2015:Dec.)
• Page Start: 2753
• Page End: 2759
• Publication Date: 2015-07-14
• Subjects: berberine -- boron‐doped diamond electrode -- Argemone mexicana -- square wave voltammetry; Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.201500356
• Languages: English
• ISSNs: 1040-0397

Abstract

Abstract: Determination of berberine, an isoquinoline plant alkaloid, with antibacterial, antiparasitic, antifungal, hypotensive and antitumoral effects, was proposed by introducing square wave voltammetry on boron‐doped diamond electrode. At optimized experimental parameters, in Britton‐Robinson buffer solution pH 5 berberine provides 3 oxidation peaks (+0.63; +1.14 and +1.34 V) and one reduction (+0.15 V) ( vs. Ag/AgCl electrode), with good repeatability (relative standard deviation of 2.6 % and 1.9 % for 8 measurements at 0.5 and 10 µM concentration level, respectively). Calibration curve was linear in wade linear range from 0.1 to 50 µM with limit of detection of 0.04 µM. The proposed procedure was successfully applied for the determination of berberine in seed extract from Argemone mexicana with satisfactory recovery (102–102.6 %). The developed method may represent a sensitive alternative to highly toxic mercury electrodes, modified electrodes and chromatographic methods. Abstract :