Fine mapping and photosynthetic characteristics of the lower chlorophyll b 1 mutant in rice (Oryza sativa L.). Issue 6 (26th October 2015)

• Record Type: Journal Article
• Authors: Liu, Jin; Wang, Jiayu; Yao, Xiaoyu; Dong, Xiaoyan; Chen, Wenfu
• Is Part Of: Plant breeding. Volume 134:Issue 6(2015)
• Page Start: 661
• Page End: 667
• Publication Date: 2015-10-26
• Subjects: rice (Oryza sativa L.) -- lower chlorophyll b -- photosynthetic -- fine mapping; Plant breeding -- Periodicals; 631.52
• DOI: 10.1111/pbr.12320
• Languages: English
• ISSNs: 0179-9541

Abstract

Abstract: Chlorophylls absorb and transfer light energy to the photosynthetic system. Consequently, chlorophyll content is strongly related to crop biomass and yield. We isolated a rice spontaneous mutant, lower chlorophyll b 1 ( lcb1 ), from a recombinant inbred line population. Under normal growth conditions, lcb1 plants produced yellow leaves with decreased total chlorophyll and chlorophyll b contents, but normal chlorophyll a content. Photosynthetic and fluorescence parameters differed between wild‐type and lcb1 plants. Compared with wild type, lcb1 had a higher electron transfer rate, a lower photochemical quenching coefficient and significantly reduced grain number, biomass and yield. A recessive nuclear gene controlled the mutant trait. Through map‐based cloning, we located the LCB1 gene in a 117.4‐kb region on the short arm of chromosome 3, close to the centromere, in a region containing 15 predicted candidate genes. None of these genes was directly related to chlorophylls or the chloroplast; therefore, lcb1 may be a mutation of a novel gene. These results will be useful for further research on the molecular mechanisms controlling biogenesis and chloroplast biochemical processes.