Muscle enzyme and fiber type‐specific sarcomere protein increases in serum after inertial concentric‐eccentric exercise. Issue 6 (2nd December 2014)

• Record Type: Journal Article
• Authors: Carmona, G.; Guerrero, M.; Cussó, R.; Padullés, J.M.; Moras, G.; Lloret, M.; Bedini, J.L.; Cadefau, J.A.
• Is Part Of: Scandinavian journal of medicine & science in sports. Volume 25:Issue 6(2015)
• Page Start: e547
• Page End: e557
• Publication Date: 2014-12-02
• Subjects: Inertial exercise -- exercise‐induced muscle damage -- knee extensor -- fast myosin -- creatine kinase -- dynamic force‐generating capacity; Sports medicine -- Periodicals; Sports -- Physiological aspects -- Periodicals; Sports -- Periodicals; 617.1027
• DOI: 10.1111/sms.12363
• Languages: English
• ISSNs: 0905-7188

Abstract

Abstract : Muscle damage induced by inertial exercise performed on a flywheel device was assessed through the serum evolution of muscle enzymes, interleukin 6, and fiber type‐specific sarcomere proteins such as fast myosin (FM) and slow myosin (SM). We hypothesized that a model of muscle damage could be constructed by measuring the evolution of serum concentration of muscle proteins following inertial exercise, according to their molecular weight and the fiber compartment in which they are located. Moreover, by measuring FM and SM, the type of fibers that are affected could be assessed. Serum profiles were registered before and 24, 48, and 144 h after exercise in 10 healthy and recreationally active young men. Creatine kinase (CK) and CK‐myocardial band isoenzyme increased in serum early (24 h) and returned to baseline values after 48 h. FM increased in serum late (48 h) and remained elevated 144 h post‐exercise. The increase in serum muscle enzymes suggests increased membrane permeability of both fast and slow fibers, and the increase in FM reveals sarcomere disruption as well as increased membrane permeability of fast fibers. Consequently, FM could be adopted as a fiber type‐specific biomarker of muscle damage.