Renal glucose release during hypoglycemia is partly controlled by sympathetic nerves – a study in pigs with unilateral surgically denervated kidneys. Issue 11 (12th November 2015)

• Record Type: Journal Article
• Authors: Bischoff, Sabine J.; Schmidt, Martin; Lehmann, Thomas; Schwab, Matthias; Matziolis, Georg; Saemann, Alexander; Schiffner, René
• Is Part Of: Physiological reports. Volume 3:Issue 11(2015:Nov.)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2015-11-12
• Subjects: Glucose release -- hypoglycemia -- renal denervation -- side dependent; Physiology -- Periodicals; 571
• DOI: 10.14814/phy2.12603
• Languages: English
• ISSNs: 2051-817X

Abstract

Abstract: Catecholamines are known to increase renal glucose release during hypoglycemia. The specific extent of the contribution of different sources of catecholamines, endocrine delivery via circulation or release from autonomous sympathetic renal nerves, though, is unknown. We tested the hypothesis that sympathetic renal innervation plays a major role in the regulation of renal gluconeogenesis. For this purpose, instrumented adolescent pigs had one kidney surgically denervated while the other kidney served as a control. A hypoglycemic clamp with arterial blood glucose below 2 mmol/L was maintained for 75 min. Arteriovenous blood glucose difference, inulin clearance, p ‐aminohippurate clearance, and sodium excretion were measured in intervals of 15 min separately for both kidneys. Blood glucose was lowered to 0.84 ± 0.33 mmol/L for 75 min. The side‐dependent renal net glucose release (SGN) decreased significantly after the unilateral ablation of renal nerves. In the linear mixed model, renal denervation had a significant inhibitory effect on renal net glucose release ( P = 0.036). The SGN of the ablated kidney decreased by 0.02 mmol/min and was equivalent to 43.3 ± 23.2% of the control (nonablated) kidney in the pigs. This allows the conclusion that renal glucose release is partly controlled by sympathetic nerves. This may be relevant in humans as well, and could explain the increased risk of severe hypoglycemia of patients with diabetes mellitus and autonomous neuropathy. The effects of denervation on renal glucose metabolism should be critically taken into account when considering renal denervation as a therapy in diabetic patients. Abstract : Instrumented adolescent pigs had one kidney surgically denervated for the purpose of testing the hypothesis whether renal innervation is a main contributing factor in the regulation of renal gluconeogenesis. The results of the experiments allow the conclusion that renal glucose release is partly controlled by sympathetic nerves. These findings could have a translational meaning for human medicine, as they reveal a probable side effect of the therapeutic use of renal denervation.