Neonatal Nicotine Exposure Leads to Hypothalamic Gliosis in Adult Overweight Rats. (December 2015)

• Record Type: Journal Article
• Authors: Younes‐Rapozo, V.; Moura, E. G.; Manhães, A. C.; Pinheiro, C. R.; Carvalho, J. C.; Barradas, P. C.; de Oliveira, E.; Lisboa, P. C.
• Is Part Of: Journal of neuroendocrinology. Volume 27:Number 12(2015:Dec.)
• Page Start: 887
• Page End: 898
• Publication Date: 2015-12
• Subjects: nicotine -- lactation -- developmental plasticity -- hypothalamus -- obesity -- inflammation; Neuroendocrinology -- Periodicals; 616.4
• DOI: 10.1111/jne.12328
• Languages: English
• ISSNs: 0953-8194

Abstract

Abstract : Astrocytes and microglia, the immune competent cells of central nercous system, can be activated in response to metabolic signals such as obesity and hyperleptinaemia. In rats, maternal exposure to nicotine during lactation leads to central obesity, hyperleptinaemia, leptin resistance and alterations in hypothalamic neuropeptides in the offspring during adulthood. In the present study, we studied the activation of astrocytes and microglia, as well as the pattern of inflammatory mediators, in adult offspring of this experimental model. On postnatal day 2 (P2), osmotic minipumps releasing nicotine (NIC) (–6 mg/kg/day) or saline for 14 days weres.c . implanted in dams. Male offspring were killed on P180 and hypothalamic immunohistochemistry, retroperitoneal white adipose tissue (WAT) polymerase chain reaction analysis and multiplex analysis for plasma inflammatory mediators were carried out. At P180, NIC astrocyte cell number was higher in the arcuate nucleus (ARC) (medial: +82%; lateral: +110%), in the paraventricular nucleus (PVN) (+144%) and in the lateral hypothalamus (+121%). NIC glial fibrillary acidic protein fibre density was higher in the lateral ARC (+178%) and in the PVN (+183%). Interleukin‐6 was not affected in the hypothalamus. NIC monocyte chemotactic protein 1 was only higher in the periventricular nucleus (+287%). NIC microglia (iba‐1‐positive) cell number was higher (+68%) only in the PVN, as was the chemokine (C‐X3‐C motif) receptor 1 density (+93%). NIC interleukin‐10 was lower in the WAT (−58%) and plasma (−50%). Thus, offspring of mothers exposed to nicotine during lactation present hypothalamic astrogliosis at adulthood and microgliosis in the PVN.