Overexpression of potato miR482e enhanced plant sensitivity to Verticillium dahliae infection. Issue 12 (24th April 2015)

• Record Type: Journal Article
• Authors: Yang, Liu; Mu, Xiaoying; Liu, Chao; Cai, Jinghui; Shi, Ke; Zhu, Wenjiao; Yang, Qing
• Is Part Of: Journal of integrative plant biology. Volume 57:Issue 12(2015)
• Page Start: 1078
• Page End: 1088
• Publication Date: 2015-04-24
• Subjects: miR482 -- nucleotide binding site leucine‐rich repeat -- potato -- sRNA -- ta‐siRNA -- Verticillium dahliae -- Verticillium wilt; Plants -- Periodicals; Plants -- China -- Periodicals; Electronic journals; 580.5
• DOI: 10.1111/jipb.12348
• Languages: English
• ISSNs: 1672-9072

Abstract

Abstract: Verticillium wilt of potato is caused by the fungus pathogen Verticillium dahliae . Present sRNA sequencing data revealed that miR482 was in response to V. dahliae infection, but the function in potato is elusive. Here, we characterized potato miR482 family and its putative role resistance to Verticillium wilt. Members of the potato miR482 superfamily are variable in sequence, but all variants target a class of disease‐resistance proteins with nucleotide binding site (NBS) and leucine‐rich repeat (LRR) motifs. When potato plantlets were infected with V. dahliae, the expression level of miR482e was downregulated, and that of several NBS‐LRR targets of miR482e were upregulated. Transgenic potato plantlets overexpressing miR482e showed hypersensitivity to V. dahliae infection. Using sRNA and degradome datasets, we validated that miR482e targets mRNAs of NBS‐LRR disease‐resistance proteins and triggers the production of trans‐acting (ta)‐siRNAs, most of which target mRNAs of defense‐related proteins. Thus, the hypersensitivity of transgenic potato could be explained by enhanced miR482e and miR482e‐derived ta‐siRNA‐mediated silencing on NBS‐LRR‐disease‐resistance proteins. It is speculated that a miR482‐mediated silencing cascade mechanism is involved in regulating potato resistance against V. dahliae infection and could be a counter defense action of plant in response to pathogen infection. Abstract : Verticillium dalihae is the pathogen of Verticillium wilt, which spreads worldwide and causes serious economic loss for many cultivars. Here we found a potato miRNA, stu‐miR482, which targets disease resistance proteins by silencing cascade, presenting a counter defense action of potato in response to Verticillium dahliae infection.