Low‐oxygen tensions found in Salmonella‐infected gut tissue boost Salmonella replication in macrophages by impairing antimicrobial activity and augmenting Salmonella virulence. (16th July 2015)

• Record Type: Journal Article
• Authors: Jennewein, Jonas; Matuszak, Jasmin; Walter, Steffi; Felmy, Boas; Gendera, Kathrin; Schatz, Valentin; Nowottny, Monika; Liebsch, Gregor; Hensel, Michael; Hardt, Wolf‐Dietrich; Gerlach, Roman G.; Jantsch, Jonathan
• Is Part Of: Cellular microbiology. Volume 17:Number 12(2015:Dec.)
• Page Start: 1833
• Page End: 1847
• Publication Date: 2015-07-16
• Subjects: Microbiology -- Periodicals; Cytology -- Periodicals; Host-parasite relationships -- Periodicals; Microbiology -- Periodicals; Cells -- Periodicals; Microbiologie -- Périodiques; Microbiologie; Relation hôte-parasite; Cytologie; Cellule; Réponse cellulaire; Ressource Internet (Descripteur de forme); Périodique électronique (Descripteur de forme); 579.05
• DOI: 10.1111/cmi.12476
• Languages: English
• ISSNs: 1462-5814

Abstract

Summary: In Salmonella infection, the S almonella pathogenicity island‐2 (SPI‐2)‐encoded type three secretion system (T3SS2) is of key importance for systemic disease and survival in host cells. For instance, in the streptomycin‐pretreated mouse model SPI‐2‐dependent S almonella replication in lamina propria CD11c − CXCR1 − monocytic phagocytes/macrophages (MΦ) is required for the development of colitis. In addition, containment of intracellular S almonella in the gut critically depends on the antimicrobial effects of the phagocyte NADPH oxidase (PHOX), and possibly type 2 nitric oxide synthase (NOS2). For both antimicrobial enzyme complexes, oxygen is an essential substrate. However, the amount of available oxygen upon enteroinvasive S almonella infection in the gut tissue and its impact on S almonella– MΦ interactions was unknown. Therefore, we measured the gut tissue oxygen levels in a model of S almonella enterocolitis using luminescence two‐dimensional in vivo oxygen imaging. We found that gut tissue oxygen levels dropped from ∼78 Torr (∼11% O2 ) to values of ∼16 Torr (∼2% O2 ) during infection. Because in vivo virulence of S almonella depends on the S almonella survival in MΦ, S almonella –MΦ interaction was analysed under such low oxygen values. These experiments revealed an increased intracellular replication and survival of wild‐type and t3ss2 non‐expressing S almonella . These findings were paralleled by blunted nitric oxide and reactive oxygen species (ROS) production and reduced S almonella ROS perception. In addition, hypoxia enhanced SPI‐2 transcription and translocation of SPI‐2‐encoded virulence protein. Neither pharmacological blockade of PHOX and NOS2 nor impairment of T3SS2 virulence function alone mimicked the effect of hypoxia on S almonella replication under normoxic conditions. However, if t3ss2 non‐expressing S almonella were used, hypoxia did not further enhance S almonella recovery in a PHOX and NOS2‐deficient situation. Hence, these data suggest that hypoxia‐induced impairment of antimicrobial activity and S almonella virulence cooperate to allow for enhanced S almonella replication in MΦ.