GLUT4 translocation is not impaired after acute exercise in skeletal muscle of women with obesity and polycystic ovary syndrome. (16th September 2015)

• Record Type: Journal Article
• Authors: Silva Dantas, Wagner; Antonio Miguel Marcondes, José; Katsuyuki Shinjo, Samuel; Augusto Perandini, Luiz; Olzon Zambelli, Vanessa; Das Neves, Willian; Roberto Grimaldi Barcellos, Cristiano; Patrocínio Rocha, Michele; Dos Reis Vieira Yance, Viviane; Tavares Dos Santos Pereira, Renato; Hisashi Murai, Igor; Lucia De Sá Pinto, Ana; Roschel, Hamilton; Gualano, Bruno
• Is Part Of: Obesity. Volume 23:Number 11(2015:Nov.)
• Page Start: 2207
• Page End: 2215
• Publication Date: 2015-09-16
• Subjects: Obesity -- Periodicals; 616.398005
• DOI: 10.1002/oby.21217
• Languages: English
• ISSNs: 1930-7381

Abstract

Abstract : Objective: The aim of this study was to examine the effects of acute exercise on insulin signaling in skeletal muscle of women with polycystic ovary syndrome (PCOS) and controls (CTRL). Methods: Fifteen women with obesity and PCOS and 12 body mass index‐matched CTRL participated in this study. Subjects performed a 40‐min single bout of exercise. Muscle biopsies were performed before and 60 min after exercise. Selected proteins were assessed by Western blotting. Results: CTRL, but not PCOS, showed a significant increase in PI3‐k p85 and AS160 Thr 642 after a single bout of exercise ( P = 0.018 and P = 0.018, respectively). Only PCOS showed an increase in Akt Thr 308 and AMPK phosphorylation after exercise ( P = 0.018 and P = 0.018, respectively). Total GLUT4 expression was comparable between groups ( P > 0.05). GLUT4 translocation tended to be significantly higher in both groups after exercise (PCOS: P = 0.093; CTRL: P = 0.091), with no significant difference between them ( P > 0.05). Conclusions: A single bout of exercise elicited similar GLUT4 translocation in skeletal muscle of PCOS and CTRL, despite a slightly differential pattern of protein phosphorylation. The absence of impairment in GLUT4 translocation suggests that PCOS patients with obesity and insulin resistance may benefit from exercise training.