Chemical Analysis in the Corpus Callosum Following Traumatic Axonal Injury using Fourier Transform Infrared Microspectroscopy: A Pilot Study1. Issue 6 (14th August 2015)

• Record Type: Journal Article
• Authors: Zhang, Ji; Liu, Liang; Mu, Jiao; Yang, Tiantong; Zheng, Na; Dong, Hongmei
• Is Part Of: Journal of forensic sciences. Volume 60:Issue 6(2015)
• Page Start: 1488
• Page End: 1494
• Publication Date: 2015-08-14
• Subjects: forensic science -- forensic pathology -- traumatic axonal injury -- traumatic brain injury -- Fourier transform infrared microspectroscopy -- animal study; Medical jurisprudence -- Periodicals; Forensic sciences -- Periodicals; Forensic Medicine -- Periodicals; Gerechtelijke geneeskunde; Gerechtelijke chemie; Gerechtelijke psychiatrie; 363.2505
• DOI: 10.1111/1556-4029.12871
• Languages: English
• ISSNs: 0022-1198

Abstract

Abstract: Evaluating traumatic axonal injury remains challenging in clinical and forensic sciences as its identification is difficult using routine diagnostic methods. This study used Fourier transform infrared microspectroscopy to detect TAI within the corpus callosum in an animal model. Protein conformational analysis revealed significantly increased β‐sheet and β‐turn contents paralleled by a decrease in α‐helix content at 24 h postinjury, while the antiparallel β‐sheet content was decreased at 12 h postinjury. Compared with the control group, the lipid/protein ratio was significantly reduced in all of the injured groups. At 24 h postinjury, there were increases in the olefinic=CH and CH3 group of lipids accompanied by the decreased CH2 group, but the results at 12 and 72 h were contrary to that at 24 h. Our study showed that FTIRM could differentiate injured from normal white matter at different time points following TBI via examination of these infrared spectral parameters.