Establishment and characterization of an orthotopic sinonasal squamous cell carcinoma mouse model. Issue 12 (25th September 2014)

• Record Type: Journal Article
• Authors: Costales, María; López, Fernando; García–Inclán, Cristina; Fernández, Soledad; Marcos, César Álvarez; Llorente, José Luis; Hermsen, Mario A.
• Is Part Of: Head & neck. Volume 37:Issue 12(2015:Dec.)
• Page Start: 1769
• Page End: 1775
• Publication Date: 2014-09-25
• Subjects: sinonasal squamous cell carcinoma -- orthotopic mouse model -- tumor invasion -- immunohistochemistry -- genetic profiling; Head -- Diseases -- Periodicals; Neck -- Diseases -- Periodicals; Head -- Periodicals; Neck -- Periodicals; Face -- Periodicals; 617.51059
• DOI: 10.1002/hed.23832
• Languages: English
• ISSNs: 1043-3074

Abstract

Abstract: Background: Despite therapeutic improvements, patients with sinonasal squamous cell carcinoma (SCC) still face an unfavorable prognosis and there is great need for alternative treatments. Methods: SCCNC4 cells, originally derived from a T2N1M0 primary and untreated sinonasal SCC, were inoculated in the maxillary sinus of immunodeficient mice. Histology, invasive behavior, and genetic features were evaluated and compared with the original primary tumor. Results: The mice developed tumors that invaded bone, surrounding tissues, and brain, showing the same poor differentiation as the original primary tumor. Genetic analysis revealed an almost identical pattern of copy number alterations, except for the deletion and loss of expression of the genes CDKN2A and PTEN . Conclusion: This article shows the feasibility of an orthotopic mouse model of SCC of the maxillary sinus. Completed by genome‐wide genetic profiling data, this model will be useful for preclinical testing of specific gene‐targeted anticancer drugs. © 2014 Wiley Periodicals, Inc. Head Neck 37: 1769–1775, 2015