Modular Integration of Upconverting Nanocrystal–Dendrimer Composites for Folate Receptor‐Specific NIR Imaging and Light‐Triggered Drug Release. Issue 45 (13th October 2015)

• Record Type: Journal Article
• Authors: Wong, Pamela T.; Chen, Dexin; Tang, Shengzhuang; Yanik, Sean; Payne, Michael; Mukherjee, Jhindan; Coulter, Alexa; Tang, Kenny; Tao, Ke; Sun, Kang; Baker, James R.; Choi, Seok Ki
• Is Part Of: Small. Volume 11:Issue 45(2015)
• Page Start: 6078
• Page End: 6090
• Publication Date: 2015-10-13
• Subjects: folate receptors -- NIR detection -- PAMAM dendrimers -- photocaged doxorubicin -- upconversion -- nanocrystals -- imaging; Nanotechnology -- Periodicals; Nanoparticles -- Periodicals; Microtechnology -- Periodicals; 620.5
• DOI: 10.1002/smll.201501575
• Languages: English
• ISSNs: 1613-6810

Abstract

Abstract : Upconversion nanocrystals (UCNs) display near‐infrared (NIR)‐responsive photoluminescent properties for NIR imaging and drug delivery. The development of effective strategies for UCN integration with other complementary nanostructures for targeting and drug conjugation is highly desirable. This study reports on a core/shell‐based theranostic system designed by UCN integration with a folate (FA)‐conjugated dendrimer for tumor targeting and with photocaged doxorubicin as a cytotoxic agent. Two types of UCNs (NaYF4 :Yb/Er (or Yb/Tm); diameter = ≈50 to 54 nm) are described, each displaying distinct emission properties upon NIR (980 nm) excitation. The UCNs are surface modified through covalent attachment of photocaged doxorubicin (ONB‐Dox) and a multivalent FA‐conjugated polyamidoamine (PAMAM) dendrimer G5(FA)6 to prepare UCN@(ONB‐Dox)(G5FA). Surface plasmon resonance experiments performed with G5(FA)6 dendrimer alone show nanomolar binding avidity ( K D = 5.9 × 10 −9 m ) to the folate binding protein. This dendrimer binding corresponds with selective binding and uptake of UCN@(ONB‐Dox)(G5FA) by FAR‐positive KB carcinoma cells in vitro . Furthermore, UCN@(ONB‐Dox)(G5FA) treatment of FAR(+) KB cells inhibits cell growth in a light dependent manner. These results validate the utility of modularly integrated UCN‐dendrimer nanocomposites for cell type specific NIR imaging and light‐controlled drug release, thus serving as a new theranostic system. Abstract : An NIR‐imagable therapeutic nano­material is designed through core–shell integration of upconverting nanocrystals with folate‐conjugated dendrimers and photocaged doxorubicin. This nanomaterial displays folate receptor‐specific tumor cell targeting and NIR detection capability. Light‐triggered drug release leads to actively controlled induction of cytotoxicity in KB tumor cells, validating this nanomaterial as a novel theranostic system.