Ex Vivo and In Situ Evaluation of 'Dispelling‐Wind' Chinese Medicine Herb–Drugs on Intestinal Absorption of Chlorogenic Acid. (30th October 2015)

• Record Type: Journal Article
• Authors: Zhai, Lixiang; Shi, Jun; Xu, Weitong; Heinrich, Michael; Wang, Jianying; Deng, Wenji
• Is Part Of: Phytotherapy research. Volume 29:Number 12(2015:Dec.)
• Page Start: 1974
• Page End: 1981
• Publication Date: 2015-10-30
• Subjects: chlorogenic acid -- Angelica dahurica -- Saposhnikovia divaricata -- intestinal absorption -- everted rat gut sac -- single‐pass intestinal perfusion; Materia medica, Vegetable -- Periodicals; Botany, Medical -- Periodicals; Medicinal plants -- Periodicals; Plant Extracts -- therapeutic use -- Periodicals; Plants, Medicinal -- Periodicals; 581.634
• DOI: 10.1002/ptr.5492
• Languages: English
• ISSNs: 0951-418X

Abstract

Abstract : This study aims to investigate the additive or synergistic effects and mechanism of intestinal absorption of extracts from two commonly used 'dispelling‐wind' TCM botanical drugs [roots of Angelica dahurica (Hoffm.) Benth. & Hook. f. ex Franch. & Sav. (RAD) and Saposhnikovia divaricata (Turcz.) Schischk. (RSD)] using chlorogenic acid as a marker substance. Ex vivo everted intestinal sac and in situ single pass perfusion methods using rats were employed to investigate the effects of two TCM botanical drugs extracts on the intestinal absorption of chlorogenic acid. Both the extracts of RAD and RSD showed synergistic properties on the intestinal absorption of chlorogenic acid. The verapamil (a P‐gp inhibitor) and intestinal dysbacteriosis model induced by norfloxacin increased the Papp and Ka of intestinal absorption of chlorogenic acid. These synergistic effects on intestinal absorption in a rat model can be correlated with the inhibition of P‐gp and regulation of gut microbiota. This experimental approach has helped to better understand changes in the absorption of chlorogenic acid under different conditions. Copyright © 2015 John Wiley & Sons, Ltd.