Highly Sensitive Voltammetric Thrombin Aptamer Sensor Based on the Synergistic Effect of Doping/Depositing Gold Nanoparticles in Polydopamine Film. Issue 11 (15th July 2015)

• Record Type: Journal Article
• Authors: Jeong, Bongjin; Akter, Rashida; Choi, Jong‐Soon; Rahman, Md. Aminur
• Is Part Of: Electroanalysis. Volume 27:Issue 11(2015:Nov.)
• Page Start: 2588
• Page End: 2595
• Publication Date: 2015-07-15
• Subjects: Voltammetric sensor -- Aptamer -- Human α‐thrombin -- Polydopamine -- Gold nanoparticles; Electrochemical analysis -- Periodicals; Chemistry, Analytical -- Periodicals; Electrochemistry -- Periodicals; 543.4
• DOI: 10.1002/elan.201500271
• Languages: English
• ISSNs: 1040-0397

Abstract

Abstract: A highly sensitive square‐wave voltammetric thrombin (TB) aptamer sensor was developed using functional polydopamine (PD) film by doping and depositing gold nanoparticles into the bulk and the surface of PD. The aptamer sensor was fabricated by immobilizing a thiolated TB‐binding aptamer (TBA) on the AuNPs‐doped/deposited PD film. AuNPs‐supported methylene blue labels were used for the detection of human α‐TB. Under the optimized conditions, the aptamer sensor's dynamic range and the detection limit were determined to be 2.0 pM–50 nM and 0.97±0.06 pM, respectively. Finally, the proposed aptamer sensor was successfully examined in human serum samples and satisfactory results were obtained. Abstract :