Cover Picture: Protein‐Specific Imaging of O‐GlcNAcylation in Single Cells (ChemBioChem 18/2015). Issue 18 (December 2015)

• Record Type: Journal Article
• Authors: Lin, Wei; Gao, Ling; Chen, Xing
• Is Part Of: Chembiochem. Volume 16:Issue 18(2015)
• Page Start: 2541
• Page End: 2541
• Publication Date: 2015-12
• Subjects: FLIM -- FRET -- glycan imaging -- O-GlcNAc -- protein labeling; Biochemistry -- Periodicals; Molecular biology -- Periodicals; Pharmaceutical chemistry -- Periodicals; 572
• DOI: 10.1002/cbic.201500635
• Languages: English
• ISSNs: 1439-4227

Abstract

Abstract : The cover picture shows a FLIM‐FRET‐based strategy for specifically imaging the O‐GlcNAcylation state of a protein of interest (POI) inside a cell where thousands of proteins are modified with O‐GlcNAc. The POI is genetically tagged with EGFP, and the O‐GlcNAc on the POI is tagged with the TAMRA fluorophore by metabolic glycan labeling. EGFP and TAMRA form a FRET pair, and the occurrence of intramolecular FRET results in the shortening of the EGFP fluorescence lifetime ( τ ); this is detected by time‐ correlated single‐photon counting. The stringent nanometer proximity requirement of FRET enables specific imaging of the O‐GlcNAcylation state of the target protein.