Detecting Cytosolic Peptide Delivery with the GFP Complementation Assay in the Low Micromolar Range. (30th October 2015)

• Record Type: Journal Article
• Authors: Schmidt, Samuel; Adjobo‐Hermans, Merel J. W.; Wallbrecher, Rike; Verdurmen, Wouter P. R.; Bovée‐Geurts, Petra H. M.; van Oostrum, Jenny; Milletti, Francesca; Enderle, Thilo; Brock, Roland
• Is Part Of: Angewandte Chemie. Volume 127:Number 50(2015)
• Page Start: 15320
• Page End: 15323
• Publication Date: 2015-10-30
• Subjects: Fragmentvervollständigung -- Lipidmembranen -- Wirkstofftransport -- Zellaufnahme -- Zellgängige Peptide; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201505913
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: Transfection of cells with a plasmid encoding for the first ten strands of the GFP protein (GFP1‐10) provides the means to detect cytosolic peptide import at low micromolar concentrations. Cytosolic import of the eleventh strand of the GFP protein either by electroporation or by cell‐penetrating peptide‐mediated import leads to formation of the full‐length GFP protein and fluorescence. An increase in sensitivity is achieved through structural modifications of the peptide and the expression of GFP1‐10 as a fusion protein with mCherry.