Carbon concentration and oxygen availability affect lipid and carotenoid production by carob pulp syrup‐grown Rhodosporidium toruloides NCYC 921. Issue 8 (25th August 2015)

• Record Type: Journal Article
• Authors: Parreira, Teresa Margarida; Freitas, Claudia; Reis, Alberto; Roseiro, José; da Silva, Teresa Lopes
• Is Part Of: Engineering in life sciences. Volume 15:Issue 8(2015)
• Page Start: 815
• Page End: 823
• Publication Date: 2015-08-25
• Subjects: Carbon concentration -- Carotenoids -- Lipids -- Oxygen availability -- Yeast; Bioengineering -- Periodicals; 660.605
• DOI: 10.1002/elsc.201500002
• Languages: English
• ISSNs: 1618-0240

Abstract

Abstract : The simultaneous effect of oxygen availability and carbon source concentration on yeast lipid and carotenoid production has never been studied before. In this work, a Doehlert distribution design was used to study the simultaneous effect of carbon concentration and oxygen availability on Rhodosporidium toruloides NCYC 921 carotenoid and lipid production. A cheap industrial byproduct was used as carbon source (carob pulp syrup). A total sugar concentration of 106.3 g/L and a medium volume of 0.120 L induced the highest total carotenoid and total fatty acid productivities (4.60 μg/Lh and 0.029 g/Lh, respectively). Flow cytometry was used to assess yeast stress response under different cultivation conditions. The highest proportion of cells with permeabilised membrane (>20%) was induced when the cultivations were carried out at the highest sugar concentration studied (130.0 g/L) or when the culture reached the minimum final medium pH (4.60). The results showed that the total sugar concentration had a positive influence on the yeast biomass and carotenoid content, while the oxygen availability had little influence on the biomass concentration, but had a slight positive influence on the carotenoid content. Regarding the fatty acids, the two factors had a negative impact on the synthesis of these compounds.