Cloning, expression, crystallization and crystallographic analysis of CouR from Rhodopseudomonas palustris. Issue 11 (1st November 2015)

• Record Type: Journal Article
• Authors: Pan, Chen; Hu, Yong‐lin; Jiang, Xiang‐ning; Gai, Ying
• Is Part Of: Acta crystallographica. Volume 71:Issue 11(2015:Nov.)
• Page Start: 1416
• Page End: 1420
• Publication Date: 2015-11-01
• Subjects: MarR family -- transcriptional regulator -- protein–ligand complex -- crystallographic study -- lignin -- p‐coumarate; Crystallography -- Periodicals; Crystals -- Periodicals; 548
• DOI: 10.1107/S2053230X15018968
• Languages: English
• ISSNs: 2053-230X

Abstract

Abstract : CouR from Rhodopseudomonas palustris is a member of the MarR transcriptional regulator family. It regulates the expression of CouA and CouB, enzymes that are involved in the degradation of p ‐coumarate. In vivo, CouR binds to a DNA fragment containing the couAB promoter and suppresses the expression of CouA and CouB, while binding of p ‐coumaroyl‐CoA attenuates its affinity towards DNA and activates the expression of CouA and CouB. Here, the crystallization and X‐ray diffraction analyses of CouR alone and in complex with p ‐coumaroyl‐CoA are reported. Apo and ligand‐complexed CouR crystals diffracted to 2.5 and 3.3 Å resolution, respectively. The crystals of apo CouR belonged to space group P 221 21, with unit‐cell parameters a = 62.78, b = 76.15, c = 87.38 Å, whereas the crystals of the CouR–ligand complex belonged to space group P 21 21 21, with unit‐cell parameters a = 61.37, b = 69.82, c = 70.32 Å. The crystals were predicted to contain two CouR molecules or CouR–ligand complexes per asymmetric unit.