Analysis of EV71 infection progression using triple‐SILAC‐based proteomics approach. Issue 21 (28th September 2015)

• Record Type: Journal Article
• Authors: Li, Hao‐Yu; Zhang, Lei‐Ke; Zhu, Xiu‐Juan; Shang, Jun; Chen, Xi; Zhu, Ying; Guo, Lin
• Is Part Of: Proteomics. Volume 15:Issue 21(2015:Nov.)
• Page Start: 3629
• Page End: 3643
• Publication Date: 2015-09-28
• Subjects: Proteins -- Separation -- Periodicals; Bioinformatics -- Periodicals; Proteomics -- Periodicals; Genomes -- Periodicals; Molecular genetics -- Periodicals; 572.605
• DOI: 10.1002/pmic.201500180
• Languages: English
• ISSNs: 1615-9853

Abstract

<abstract abstract-type="main"> <title> <x xml:space="preserve">Abstract</x> </title> <p>Enterovirus 71 (EV71), a member of <italic>Picornaviridae</italic>, causes severe neurological and systemic illness in children. To better understand the virus–host cell interactions, we performed a triple‐SILAC‐based quantitative proteomics study monitoring host cell proteome changes after EV71 infection. Based on the quantitative data for more than 4100 proteins, ∼17% of the proteins were found as significantly changed (<italic>p</italic>&lt;0.01) at either 8 or 20 hours post infection. Five biological processes and seven protein classes showed significant differences. Functional screening of nine regulated proteins discovered the regulatory role of CHCH2, a mitochondrial protein known as a transcriptional activator for cytochrome c oxidase, in EV71 replication. Further studies showed that CHCH2 served as a negative regulator of innate immune responses. All MS data have been deposited in the ProteomeXchange with identifier PXD002483 (<ext-link ext-link-type="uri" xlink:href="http://proteomecentral.proteomexchange.org/dataset/PXD002483" xlink:type="simple" xmlns:xlink="http://www.w3.org/1999/xlink">http://proteomecentral.proteomexchange.org/dataset/PXD002483</ext-link>).</p> </abstract>