CD44 single nucleotide polymorphism and isoform switching may predict gastric cancer recurrence. Issue 6 (28th September 2015)

• Record Type: Journal Article
• Authors: Suenaga, Masaya; Yamada, Suguru; Fuchs, Bryan C.; Fujii, Tsutomu; Kanda, Mitsuro; Tanaka, Chie; Kobayashi, Daisuke; Fujiwara, Michitaka; Tanabe, Kenneth K.; Kodera, Yasuhiro
• Is Part Of: Journal of surgical oncology. Volume 112:Issue 6(2015:Nov. 01)
• Page Start: 622
• Page End: 628
• Publication Date: 2015-09-28
• Subjects: Cancer -- Surgery -- Periodicals; Neoplasms -- Periodicals; 616
• DOI: 10.1002/jso.24056
• Languages: English
• ISSNs: 0022-4790

Abstract

<abstract abstract-type="main" xml:lang="en"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="jso24056-sec-0001" sec-type="section"> <title>Background and Objectives</title> <p>The clinical implications of single nucleotide polymorphisms (SNPs) in <italic>CD44</italic> remain unclear. This study examined the relationships of <italic>CD44</italic> SNPs with clinicopathological parameters and prognosis in Japanese gastric cancer patients.</p> </sec> <sec id="jso24056-sec-0002" sec-type="section"> <title>Methods</title> <p>The <italic>CD44</italic> SNPs were analyzed in 11 gastric cancer cell lines and 517 clinical specimens. The expression of CD44 standard (CD44s) and CD44 variant 9 isoform (CD44v9) transcripts were measured by quantitative real‐time polymerase chain reaction.</p> </sec> <sec id="jso24056-sec-0003" sec-type="section"> <title>Results</title> <p>The <italic>CD44</italic> rs187116 A/A, A/G, and G/G genotypes were present in 10.3%, 45.1%, and 44.7% of patients, respectively. The presence of <italic>CD44</italic> rs187116 A/G or G/G genotypes was significantly associated with positive peritoneal washing cytology (<italic>P</italic> = 0.012). Disease‐free survival of patients with these genotypes was significantly worse than in those with the A/A genotype (<italic>P</italic> = 0.039). Multivariate analysis showed that the <italic>CD44</italic> rs187116 was independently prognostic of disease‐free survival (<italic>P</italic> = 0.047). The CD44s/CD44v9 ratio was significantly lower in patients with the <italic>CD44</italic> rs187116 A/A genotype than in those with the A/G (<italic>P</italic> = 0.046) and G/G (<italic>P</italic> = 0.047) genotypes.</p> </sec> <sec id="jso24056-sec-0004" sec-type="section"> <title>Conclusions</title> <p>The <italic>CD44</italic> rs187116 genotype could predict disease recurrence in Japanese gastric cancer patients, and the SNP was associated with CD44 isoform switching. <italic>J. Surg. Oncol. 2015;112:622–628</italic>. © 2015 Wiley Periodicals, Inc.</p> </sec> </abstract>