Antimicrobial activity of Chlorhexidine, Peracetic acid and Sodium hypochlorite/etidronate irrigant solutions against Enterococcus faecalis biofilms. (8th January 2015)

• Record Type: Journal Article
• Authors: Arias‐Moliz, M. T.; Ordinola‐Zapata, R.; Baca, P.; Ruiz‐Linares, M.; García García, E.; Hungaro Duarte, M. A.; Monteiro Bramante, C.; Ferrer‐Luque, C. M.
• Is Part Of: International endontic journal. Volume 48:Number 12(2015:Dec.)
• Page Start: 1188
• Page End: 1193
• Publication Date: 2015-01-08
• Subjects: Endodontics -- Periodicals; 617.6342
• DOI: 10.1111/iej.12424
• Languages: English
• ISSNs: 0143-2885

Abstract

<abstract abstract-type="main" id="iej12424-abs-0001"> <title>Abstract</title> <sec id="iej12424-sec-0001" sec-type="section"> <title>Aim</title> <p>To evaluate the antimicrobial effect of 2.5% sodium hypochlorite alone (NaOCl) and associated with 9% HEBP (NaOCl/HEBP), 2% peracetic acid (PAA) and 2% chlorhexidine (CHX), on the viability of <italic>Enterococcus faecalis</italic> biofilms attached to dentine.</p> </sec> <sec id="iej12424-sec-0002" sec-type="section"> <title>Methodology</title> <p>Biofilms of <italic>E. faecalis</italic> were grown on the surface of dentine blocks for 5 days and then exposed to the irrigating solutions for 3 min. Distilled water was used as the control. The total biovolume and the percentage of dead cells of the infected dentine were measured by means of confocal microscopy and the live/dead technique. Nonparametric tests were used to determine statistical differences (<italic>P </italic><<italic> </italic>0.05).</p> </sec> <sec id="iej12424-sec-0003" sec-type="section"> <title>Results</title> <p>NaOCl and the NaOCl/HEBP mixture were associated with a significantly greater percentage of dead cells, followed by PAA (<italic>P </italic><<italic> </italic>0.05). No significant antimicrobial effect of CHX was observed in comparison with the control group. Total biovolume decreased significantly in NaOCl, NaOCl/HEBP and PAA solutions in comparison with the CHX and control groups.</p> </sec> <sec id="iej12424-sec-0004" sec-type="section"> <title>Conclusions</title> <p>NaOCl alone or associated with HEBP were the most effective irrigant solutions in dissolving and killing <italic>E. faecalis</italic> biofilms.</p> </sec> </abstract>