Determination and validation of hupehenine in rat plasma by UPLC‐MS/MS and its application to pharmacokinetic study. (1st June 2015)

• Record Type: Journal Article
• Authors: Wen, Congcong; Wang, Shuanghu; Huang, Xueli; Liu, Zezheng; Lin, Yingying; Yang, Suping; Ma, Jianshe; Zhou, Yunfang; Wang, Xianqin
• Is Part Of: Biomedical chromatography. Volume 29:Number 12(2015:Dec.)
• Page Start: 1805
• Page End: 1810
• Publication Date: 2015-06-01
• Subjects: Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.3499
• Languages: English
• ISSNs: 0269-3879

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <p>In this work, a sensitive and selective ultra‐performance liquid chromatography–tandem mass spectrometry (UPLC‐MS/MS) method for determination of hupehenine in rat plasma was developed and validated. After addition of imperialine as an internal standard (IS), protein precipitation by acetonitrile–methanol (9:1, v/v) was used to prepare samples. Chromatographic separation was achieved on a UPLC BEH C₁₈ column (2.1 × 100 mm, 1.7 µm) with 0.1% formic acid and acetonitrile as the mobile phase with gradient elution. An electrospray ionization source was applied and operated in positive ion mode; multiple reaction monitoring mode was used for quantification using target fragment ions <italic>m</italic>/<italic>z</italic> 416.3 → 98.0 for hupehenine, and <italic>m</italic>/<italic>z</italic> 430.3 → 138.2 for IS. Calibration plots were linear throughout the range 2–2000 ng/mL for hupehenine in rat plasma. Mean recoveries of hupehenine in rat plasma ranged from 92.5 to 97.3%. Relative standard deviations of intra‐day and inter‐day precision were both &lt;6%. The accuracy of the method was between 92.7 and 107.4%. The method was successfully applied to a pharmacokinetic study of hupehenine after either oral or intravenous administration. For the first time, the bioavailability of hupehenine was reported as 13.4%. Copyright © 2015 John Wiley &amp; Sons, Ltd.</p> </abstract>