The brominated flame retardants TBP-AE and TBP-DBPE antagonize the chicken androgen receptor and act as potential endocrine disrupters in chicken LMH cells. Issue 8 (December 2015)

• Record Type: Journal Article
• Authors: Asnake, Solomon; Pradhan, Ajay; Kharlyngdoh, Joubert Banjop; Modig, Carina; Olsson, Per-Erik
• Is Part Of: Toxicology in vitro. Volume 29:Issue 8(2015)
• Page Start: 1993
• Page End: 2000
• Publication Date: 2015-12
• Subjects: Toxicity testing -- In vitro -- Periodicals; Toxicology -- Periodicals; 615.9
• DOI: 10.1016/j.tiv.2015.08.009
• Languages: English
• ISSNs: 0887-2333

Abstract

<abstract abstract-type="author" id="ab0005"> <title id="st0005">Abstract</title> <sec> <p id="sp0005">Increased exposure of birds to endocrine disrupting compounds has resulted in developmental and reproductive dysfunctions. We have recently identified the flame retardants, allyl-2, 4, 6-tribromophenyl ether (TBP-AE), 2-3-dibromopropyl-2, 4, 6-tribromophenyl ether (TBP-DBPE) and the TBP-DBPE metabolite 2-bromoallyl-2, 4, 6-tribromophenyl ether (TBP-BAE) as antagonists to both the human androgen receptor (AR) and the zebrafish AR. In the present study, we aimed at determining whether these compounds also interact with the chicken AR. <italic>In silico</italic> modeling studies showed that TBP-AE, TBP-BAE and TBP-DBPE were able to dock into to the chicken AR ligand-binding pocket. <italic>In vitro</italic> transfection assays revealed that all three brominated compounds acted as chicken AR antagonists, inhibiting testosterone induced AR activation. In addition, qRT-PCR studies confirmed that they act as AR antagonists and demonstrated that they also alter gene expression patterns of apoptotic, anti-apoptotic, drug metabolizing and amino acid transporter genes. These studies, using chicken LMH cells, suggest that TBP-AE, TBP-BAE and TBP-DBPE are potential endocrine disrupters in chicken.</p> </sec> </abstract>