Effect of protein adsorption on cell uptake and blood clearance of methoxy poly(ethylene glycol)‐poly(caprolactone) nanoparticles. Issue 3 (10th September 2015)

• Record Type: Journal Article
• Authors: Li, Jing; Wu, Ninghua; Wu, Jiliang; Wan, Ying; Liu, Chao
• Is Part Of: Journal of applied polymer science. Volume 133:Issue 3(2016)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2015-09-10
• Subjects: Polymers -- Periodicals; Polymerization -- Periodicals; 668.9
• DOI: 10.1002/app.42884
• Languages: English
• ISSNs: 0021-8995

Abstract

<abstract abstract-type="main"> <title>ABSTRACT</title> <p>The interactions between nanoparticles and cells or tissues are frequently mediated by different biomolecules adsorbed onto the surface of nanoparticles. In this study, several methoxy poly(ethylene glycol)‐poly(ε‐caprolactone) (mPEG‐PCL) copolymers with various mPEG/PCL ratios were synthesized and used to produce three types of mPEG‐PCL nanoparticles. The protein‐adsorption behavior of nanoparticles was assessed using fetal‐bovine‐serum (FBS) as a model protein. The cell uptake of nanoparticles at different nanoparticle doses as well as various culture periods was examined by measuring their endocytosis rate related to Hela cells cultured in FBS‐free and FBS‐contained media. The blood clearance of nanoparticles was evaluated using Kunming mice to see the differences in circulation durations of nanoparticles. Results suggest that that FBS is able to significantly regulate the cell uptake of nanoparticles <italic>in vitro</italic>, and on the other hand, the size and mPEG/PCL molar ratio of mPEG/PCL nanoparticles are closely correlated to their blood clearance. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. <bold>2016</bold>, <italic>133</italic>, 42884.</p> </abstract>