Arabidopsis PLC2 is involved in auxin‐modulated reproductive development. (November 2015)

• Record Type: Journal Article
• Authors: Li, Lin; He, Yuqing; Wang, Yarui; Zhao, Shujuan; Chen, Xi; Ye, Tiantian; Wu, Yuxuan; Wu, Yan
• Is Part Of: Plant journal. Volume 84:Number 3(2015:Nov.)
• Page Start: 504
• Page End: 515
• Publication Date: 2015-11
• Subjects: Plant molecular biology -- Periodicals; Plant cells and tissues -- Periodicals; Botany -- Periodicals; 580
• DOI: 10.1111/tpj.13016
• Languages: English
• ISSNs: 0960-7412

Abstract

<abstract abstract-type="main" id="tpj13016-abs-0001"> <title>Summary</title> <p>Phospholipase C (PLC) is an enzyme that plays crucial roles in various signal transduction pathways in mammalian cells. However, the role of PLC in plant development is poorly understood. Here we report involvement of PLC2 in auxin‐mediated reproductive development in Arabidopsis. Disruption of PLC2 led to sterility, indicating a significant role for PLC2 in reproductive development. Development of both male and female gametophytes was severely perturbed in <italic>plc2</italic> mutants. Moreover, elevated auxin levels were observed in <italic>plc2</italic> floral tissues, suggesting that the infertility of <italic>plc2</italic> plants may be associated with increased auxin concentrations in the reproductive organs. We show that expression levels of the auxin reporters DR5:GUS and DR5:GFP were elevated in <italic>plc2</italic> anthers and ovules. In addition, we found that expression of the auxin biosynthetic <italic>YUCCA</italic> genes was increased in <italic>plc2</italic> plants. We conclude that PLC2 is involved in auxin biosynthesis and signaling, thus modulating development of both male and female gametophytes in Arabidopsis.</p> </abstract>