Knudson's hypothesis revisited in Indian retinoblastoma patients. Issue 4 (12th August 2015)

• Record Type: Journal Article
• Authors: Gaikwad, Namrata; Vanniarajan, Ayyasamy; Husain, Akram; Jeyaram, Illaiyaraja; Thirumalairaj, Kannan; Santhi, Radhakrishnan; Muthukkaruppan, Veerappan; Kim, Usha
• Is Part Of: Asia-Pacific journal of clinical oncology. Volume 11:Issue 4(2015:Dec.)
• Page Start: 299
• Page End: 307
• Publication Date: 2015-08-12
• Subjects: Oncology -- Pacific Area -- Periodicals; Cancer -- Treatment -- Pacific Area -- Periodicals; Cancer -- Pacific Area -- Periodicals; Cancer -- Treatment -- Periodicals; 616.9940095
• DOI: 10.1111/ajco.12401
• Languages: English
• ISSNs: 1743-7555

Abstract

<abstract abstract-type="main"> <title>Abstract</title> <sec id="ajco12401-sec-0001" sec-type="section"> <title>Aim</title> <p>Retinoblastoma (RB) is the most common primary intraocular malignancy affecting children under 5 years of age. This study aims to correlate the clinical parameters with RB1 mutation in the light of Knudson's two‐hit hypothesis in Indian RB patients.</p> </sec> <sec id="ajco12401-sec-0002" sec-type="section"> <title>Methods</title> <p>We analyzed the clinical details of 73 RB patients visiting Aravind Eye Hospital, Madurai, India, between January and October 2012. Data on gender, presenting age and sign, laterality, number of tumors in each eye and family history were collected. A semi log plot was derived based on Knudson's two‐hit hypothesis. Genetic analysis of RB1 was carried out to identify the two hits.</p> </sec> <sec id="ajco12401-sec-0003" sec-type="section"> <title>Results</title> <p>The mean age at diagnosis for unilateral and bilateral cases was 24.0 ± 15.1 and 9.8 ± 11.5 months, respectively. Familial RB was seen in 13 (17.8%) patients of whom 11 were bilateral. Multiple tumors were observed more frequently in bilateral than in unilateral cases. All unilateral and bilateral patients followed the two‐hit and one‐hit curves, respectively, confirming Knudson's hypothesis in Indian patients. Genetic analysis identified two somatic mutations in tumor samples of sporadic unilateral cases. Among the two bilateral patients, one received the first hit from her father and the other patient developed a de novo germline mutation during early development.</p> </sec> <sec id="ajco12401-sec-0004" sec-type="section"> <title>Conclusion</title> <p>The two‐hit hypothesis has been reestablished in Indian patients. Genetic analysis of tumor samples has also complemented the statistical analysis to reaffirm the two hits in tumor development.</p> </sec> </abstract>