Calcium Carbonate Mineralized Nanoparticles as an Intracellular Transporter of Cytochrome c for Cancer Therapy. Issue 11 (26th August 2015)

• Record Type: Journal Article
• Authors: Koo, Ahn Na; Min, Kyung Hyun; Lee, Hong Jae; Jegal, Jun Ho; Lee, Jae Won; Lee, Sang Cheon
• Is Part Of: Chemistry, an Asian journal. Volume 10:Issue 11(2015:Nov.)
• Page Start: 2380
• Page End: 2387
• Publication Date: 2015-08-26
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1002/asia.201500630
• Languages: English
• ISSNs: 1861-4728

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>A new intracellular delivery system based on an apoptotic protein‐loaded calcium carbonate (CaCO₃) mineralized nanoparticle (MNP) is described. Apoptosis‐inducing cytochrome c (Cyt c) loaded CaCO₃ MNPs (Cyt c MNPs) were prepared by block copolymer mediated in situ CaCO₃ mineralization in the presence of Cyt c. The resulting Cyt c MNPs had a vaterite polymorph of CaCO₃ with a mean hydrodynamic diameter of 360.5 nm and exhibited 60 % efficiency for Cyt c loading. The Cyt c MNPs were stable at physiological pH (pH 7.4) and effectively prohibited the release of Cyt c, whereas, at intracellular endosomal pH (pH 5.0), Cyt c release was facilitated. The MNPs enable the endosomal escape of Cyt c for effective localization of Cyt c in the cytosols of MCF‐7 cells. Flow cytometry showed that the Cyt c MNPs effectively induced apoptosis of MCF‐7 cells. These findings indicate that the CaCO₃ MNPs can meet the prerequisites for delivery of cell‐impermeable therapeutic proteins for cancer therapy.</p> </abstract>