Reversible and Selective Fluorescence Detection of Histidine Using a Naphthalimide‐Based Chemosensing Ensemble. Issue 11 (20th August 2015)

• Record Type: Journal Article
• Authors: Meng, Qingtao; Jia, Hongmin; Gao, Xue; Wang, Yue; Zhang, Run; Wang, Renjie; Zhang, Zhiqiang
• Is Part Of: Chemistry, an Asian journal. Volume 10:Issue 11(2015:Nov.)
• Page Start: 2411
• Page End: 2418
• Publication Date: 2015-08-20
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1002/asia.201500690
• Languages: English
• ISSNs: 1861-4728

Abstract

<abstract abstract-type="main" xml:lang="en"> <title>Abstract</title> <p>We described a new ensemble‐approach‐based chemosensor, NCH‐Cu²⁺, for highly selective and reversible detection of histidine (His) in aqueous solution and live cells. The ligand NCH exhibited specific binding with Cu²⁺ ions over other metal ions, accompanied with a 92.2 % fluorescence quenching. The decomplexation of NCH‐Cu²⁺ ensemble by His led to the liberation of the fluorophore, NCH, and thus the fluorescence was recovered. The specific fluorescence enhancement of NCH‐Cu²⁺ towards His showed a good linearity with a detection of limit at 70 n<sc>m</sc>. Quantification of intracellular His at the single cell level was achieved by microscopy and flow cytometry. Besides the UV/Vis and emission titration, reversibility of the NCH‐Cu²⁺ towards His was further confirmed by imaging and cytometry analysis. In addition, microscopy studies revealed that NCH‐Cu²⁺ was distributed in the lysosome of live cells, where it could be employed as a fluorescent biosensor for imaging of His at subcellular level.</p> </abstract>