Similar ex vivo expansion and post-irradiation regenerative potential of juvenile and aged salivary gland stem cells. Issue 3 (September 2015)

• Record Type: Journal Article
• Authors: Maimets, Martti; Bron, Reinier; de Haan, Gerald; van Os, Ronald; Coppes, Robert P.
• Is Part Of: Radiotherapy and oncology. Volume 116:Issue 3(2015:Sep.)
• Page Start: 443
• Page End: 448
• Publication Date: 2015-09
• Subjects: Oncology -- Periodicals; Radiotherapy -- Periodicals; Tumors -- Periodicals; Medical Oncology -- Periodicals; Neoplasms -- radiotherapy -- Periodicals; Radiotherapy -- Periodicals; Radiothérapie -- Périodiques; Cancérologie -- Périodiques; Tumeurs -- Périodiques; Electronic journals; 616.9940642
• DOI: 10.1016/j.radonc.2015.06.022
• Languages: English
• ISSNs: 0167-8140

Abstract

<abstract xml:lang="en" abstract-type="author" id="ab005"> <title id="st005">Abstract</title> <sec> <title id="st010">Background and purpose</title> <p id="sp0005">Salivary gland dysfunction is a major side effect of radiotherapy for head and neck cancer patients, which in the future might be salvaged by autologous adult salivary gland stem cell (SGSC) therapy. Since frail elderly patients may have decreased activity of SGSCs, we aimed to characterize the potential of aged SGSC-population in a murine model.</p> </sec> <sec> <title id="st015">Materials and methods</title> <p id="sp0010">Salivary glands and salisphere-derived cells from young and old mice were tested for CD24 and CD29 stem cell marker expression using FACS. Moreover, <italic>in vitro</italic> expansion capability and <italic>in vivo</italic> regeneration potential upon post-irradiation transplantation of young and aged SGSCs were measured.</p> </sec> <sec> <title id="st020">Results</title> <p id="sp0015">An increase in CD24<sup>hi</sup>/CD29<sup>hi</sup> putative stem cells was detected in aged salivary glands albeit with a decrease in functional ability to form salispheres. However, the salispheres formed from aged mice salivary glands expressed CD24<sup>hi</sup>/CD29<sup>hi</sup> to the same extent as the ones from young mice. Moreover, following exposure to adequate growth conditions old and young SGSCs exhibited similar <italic>in vitro</italic> expansion- and <italic>in vivo</italic> regeneration potential.</p> </sec> <sec> <title id="st025">Conclusions</title> <p id="sp0020">Aged SGSCs although reduced in number are <italic>in vitro</italic> indistinguishable from young SGSCs and could potentially be used to ameliorate age- or treatment related salivary gland dysfunction.</p> </sec> </abstract>