Local hypoxia in oral mucosa (mouse) during daily fractionated irradiation – Effect of pentoxifylline. Issue 3 (September 2015)

• Record Type: Journal Article
• Authors: Gruber, Sylvia; Hamedinger, Daniel; Bozsaky, Eva; Schmidt, Margret; Wolfram, Kathrin; Haagen, Julia; Habelt, Bettina; Puttrich, Martin; Dörr, Wolfgang
• Is Part Of: Radiotherapy and oncology. Volume 116:Issue 3(2015:Sep.)
• Page Start: 404
• Page End: 408
• Publication Date: 2015-09
• Subjects: Oncology -- Periodicals; Radiotherapy -- Periodicals; Tumors -- Periodicals; Medical Oncology -- Periodicals; Neoplasms -- radiotherapy -- Periodicals; Radiotherapy -- Periodicals; Radiothérapie -- Périodiques; Cancérologie -- Périodiques; Tumeurs -- Périodiques; Electronic journals; 616.9940642
• DOI: 10.1016/j.radonc.2015.03.024
• Languages: English
• ISSNs: 0167-8140

Abstract

<abstract xml:lang="en" abstract-type="author" id="ab005"> <title id="st120">Abstract</title> <sec> <title id="st090">Purpose</title> <p id="sp0005">A significant reduction of radiation-induced oral mucositis by systemic application of pentoxifylline has been demonstrated in a mouse tongue model. However, the underlying mechanisms remain unclear. The present study focuses on the development of local hypoxia in mouse tongue during daily fractionated irradiation and a potential modulation by pentoxifylline.</p> </sec> <sec> <title id="st095">Materials and methods</title> <p id="sp0010">Daily fractionated irradiation with 5 × 3 Gy/week (days 0–4, 7–11) was given to the snouts of mice. Groups of 3 animals per day were sacrificed between day 0 and 14. Pentoxifylline (15 mg/kg, s.c.) was administered daily from day -5 to the day before the mice were sacrificed. The expression of intrinsic hypoxia markers HIF-1α and GLUT1 in the epithelium of the lower tongue surface was analysed by immunohistochemistry in 3 animals per day; the percentage of positive epithelial cells and the staining intensity were analysed as endpoints.</p> </sec> <sec> <title id="st100">Results</title> <p id="sp0015">Compared to untreated control tissue, fractionated irradiation resulted in a progressive increase in the expression of both hypoxia markers. This effect was significantly reduced by pentoxifylline.</p> </sec> <sec> <title id="st105">Conclusion</title> <p id="sp0020">An early onset of local hypoxia occurs during fractionated irradiation in mouse tongue epithelium. The effect is markedly reduced by the mucoprotective agent pentoxifylline, suggesting a mucositis-promoting role of hypoxia; this, however, deserves further investigation.</p> </sec> </abstract>