Acinetobacter baumannii clonal lineages I and II harboring different carbapenem-hydrolyzing-β-lactamase genes are widespread among hospitalized burn patients in Tehran. Issue 6 (November 2015)

• Record Type: Journal Article
• Authors: Mahdian, Somayeh; Sadeghifard, Nourkhoda; Pakzad, Iraj; Ghanbari, Fatemeh; Soroush, Setareh; Azimi, Lila; Rastegar-Lari, Abdolaziz; Giannouli, Maria; Taherikalani, Morovat
• Is Part Of: Journal of infection and public health. Volume 8:Issue 6(2015)
• Page Start: 533
• Page End: 542
• Publication Date: 2015-11
• Subjects: Communicable diseases -- Periodicals; Public health -- Periodicals; Epidemiology -- Periodicals; Nosocomial infections -- Prevention -- Periodicals; Medical microbiology -- Periodicals; 614.4
• DOI: 10.1016/j.jiph.2015.04.030
• Languages: English
• ISSNs: 1876-0341

Abstract

<abstract abstract-type="author" id="abs0005"> <title id="sect0005">Summary</title> <sec> <p id="spar0005">The aim of this study was to analyze antimicrobial resistance patterns and their encoding genes and genotypic diversity of <italic>Acinetobacter baumannii</italic> isolated from burn patients in Tehran, Iran. The presence of extended-spectrum beta-lactamase- and <italic>bla</italic><sub><italic>OXA</italic></sub>-encoding genes among 37 multidrug resistant (MDR) <italic>A. baumannii</italic> strains isolated from patients hospitalized in a teaching hospital in Tehran was evaluated. Susceptibility to 7 antibiotics was tested by disk agar diffusion and to polymyxin B and colistin was tested by E-test, according to CLSI guidelines. All isolates were then analyzed by PCR for the presence of <italic>bla</italic><sub><italic>IMP</italic></sub>, <italic>bla</italic><sub><italic>VIM</italic></sub>, <italic>bla</italic><sub><italic>SIM</italic></sub><italic>bla</italic><sub><italic>OXA</italic>-23</sub>, <italic>bla</italic><sub><italic>OXA</italic>-24</sub>, and <italic>bla</italic><sub><italic>OXA</italic>-58</sub>-like carbapenemase genes, and <italic>bla</italic><sub><italic>OXA</italic>-51</sub>-like, <italic>bla</italic><sub><italic>TEM</italic></sub>, <italic>bla</italic><sub><italic>SHV</italic></sub>, <italic>bla</italic><sub><italic>PER</italic></sub>, <italic>bla</italic><sub><italic>VEB</italic></sub>, and <italic>bla</italic><sub><italic>GIM</italic></sub> genes. Genotyping of <italic>A. baumannii</italic> strains was performed by repetitive sequence-based (REP)-PCR and cluster analysis of REP-PCR profiles. <italic>A. baumannii</italic> isolates were assigned to international clones by multiplex PCR sequence group analysis. Twenty-five <italic>A. baumannii</italic> isolates were classified as MDR, and 12 were classified as extensively drug resistant. All isolates were susceptible to colistin and polymyxin B. Eighty-one percent of the isolates was resistant to imipenem or meropenem and harbored at least one or both of the <italic>bla</italic><sub><italic>OXA</italic>-23</sub>-like or <italic>bla</italic><sub><italic>OXA</italic>-24</sub>-like carbapenemase genes. Co-existence of different resistance genes was found among carbapenem-resistant isolates. Multiplex PCR sequence group analysis most commonly assigned <italic>A. baumannii</italic> isolates to international clones I (18/37; 48.6%) and II (18/37; 48.6%). An alarming increase in resistance to carbapenems and the spread of <italic>bla</italic><sub><italic>OXA</italic>-23</sub>-like and/or <italic>bla</italic><sub><italic>OXA</italic>-24</sub>-like carbapenemase genes was observed among <italic>A. baumannii</italic> strains belonging to clonal lineages I and II, isolated from burn patients in Tehran.</p> </sec> </abstract>