Combining tape stripping and non‐invasive reflectance confocal microscopy : an in vivo model to study skin damage. Issue 4 (12th March 2015)

• Record Type: Journal Article
• Authors: Peppelman, M.; van den Eijnde, W. A. J.; Jaspers, E. J.; Gerritsen, M‐J. P.; van Erp, P. E. J.
• Is Part Of: Skin research and technology. Volume 21:Issue 4(2015)
• Page Start: 474
• Page End: 484
• Publication Date: 2015-03-12
• Subjects: Skin -- Research -- Periodicals; Skin -- Diseases -- Periodicals; Skin -- Physiology -- Periodicals; 616.5
• DOI: 10.1111/srt.12217
• Languages: English
• ISSNs: 0909-752X

Abstract

<abstract abstract-type="main" id="srt12217-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <sec id="srt12217-sec-0001" sec-type="section"> <title>Background</title> <p>Evaluation of (immuno)histological and cell biological changes in damaged skin requires often an invasive skin biopsy, making <italic>in vivo</italic> models inappropriate to study skin damage. Reflectance confocal microscopy (RCM) might overcome this limitation. Therefore, we evaluated the use of a tape‐stripping model in combination with RCM to provide morphological data on skin damage and recovery.</p> </sec> <sec id="srt12217-sec-0002" sec-type="section"> <title>Methods</title> <p>In 25 volunteers, a tape‐stripping stimulus was applied. The skin was imaged with RCM during 1 week and 3 mm punch biopsies were obtained.</p> </sec> <sec id="srt12217-sec-0003" sec-type="section"> <title>Results</title> <p>Strong correlations between epidermal thickness determined by RCM and conventional histological measurements were found. RCM thickness measurements correlated well with epidermal proliferation. The 10× or 15× repeated tape‐stripping resulted in skin damage similar to acute stripping. Mild repeated tape‐stripping showed no skin damage.</p> </sec> <sec id="srt12217-sec-0004" sec-type="section"> <title>Conclusion</title> <p>Overall, we demonstrated that non‐invasive RCM in combination with tape‐stripping could be used as model to obtain morphological and cell biological data on skin‐material interactions.</p> </sec> </abstract>