Role for the banana AGAMOUS‐like gene MaMADS7 in regulation of fruit ripening and quality. Issue 3 (22nd June 2015)

• Record Type: Journal Article
• Authors: Liu, Juhua; Liu, Lin; Li, Yujia; Jia, Caihong; Zhang, Jianbin; Miao, Hongxia; Hu, Wei; Wang, Zhuo; Xu, Biyu; Jin, Zhiqiang
• Is Part Of: Physiologia plantarum. Volume 155:Issue 3(2015:Nov.)
• Page Start: 217
• Page End: 231
• Publication Date: 2015-06-22
• Subjects: Plant physiology -- Periodicals; 571.2
• DOI: 10.1111/ppl.12348
• Languages: English
• ISSNs: 0031-9317

Abstract

<abstract abstract-type="main" id="ppl12348-abs-0001"> <title> <x xml:space="preserve">Abstract</x> </title> <p id="ppl12348-para-0001">MADS‐box transcription factors play important roles in organ development. In plants, most studies on MADS‐box genes have mainly focused on flower development and only a few concerned fruit development and ripening. A new MADS‐box gene named <italic>MaMADS7</italic> was isolated from banana fruit by rapid amplification of cDNA ends (RACE) based on a MADS‐box fragment obtained from a banana suppression subtractive hybridization (SSH) cDNA library. <italic>MaMADS7</italic> is an AGAMOUS‐like MADS‐box gene that is preferentially expressed in the ovaries and fruits and in tobacco its protein product localizes to the nucleus. This study found that <italic>MaMADS7</italic> expression can be induced by exogenous ethylene. Ectopic expression of <italic>MaMADS7</italic> in tomato resulted in broad ripening phenotypes. The expression levels of seven ripening and quality‐related genes, <italic>ACO1, ACS2, E4, E8, PG, CNR</italic> and <italic>PSY1</italic> in <italic>MaMADS7</italic> transgenic tomato fruits were greatly increased while the expression of the AG‐like MADS‐box gene <italic>TAGL1</italic> was suppressed. Compared with the control, the contents of β‐carotene, lycopene, ascorbic acid and organic acid in transformed tomato fruits were increased, while the contents of glucose and fructose were slightly decreased. MaMADS7 interacted with banana 1‐aminocyclopropane‐1‐carboxylic acid (ACC) oxidase gene 1 (<italic>MaACO1</italic>) and tomato phytoene synthase gene (<italic>LePSY1</italic>) promoters. Our results indicated that <italic>MaMADS7</italic> plays an important role in initiating endogenous ethylene biosynthesis and fruit ripening.</p> </abstract>