Characterization of an inducible C2H2‐type zinc finger transcription factor VuSTOP1 in rice bean (Vigna umbellata) reveals differential regulation between low pH and aluminum tolerance mechanisms. Issue 2 (13th May 2015)

• Record Type: Journal Article
• Authors: Fan, Wei; Lou, He Qiang; Gong, Yu Long; Liu, Mei Ya; Cao, Meng Jie; Liu, Yu; Yang, Jian Li; Zheng, Shao Jian
• Is Part Of: New phytologist. Volume 208:Issue 2(2015:Nov. 01)
• Page Start: 456
• Page End: 468
• Publication Date: 2015-05-13
• Subjects: Botany -- Periodicals; 580
• DOI: 10.1111/nph.13456
• Languages: English
• ISSNs: 0028-646X

Abstract

<abstract abstract-type="main" id="nph13456-abs-0001"> <title>Summary</title> <p> <list id="nph13456-list-0001" list-type="bullet"> <list-item> <p>The rice bean (<italic>Vigna umbellata</italic>) root apex specifically secretes citrate through expression activation of <italic>Vigna umbellata Multidrug and Toxic Compound Extrusion 1</italic> (<italic>VuMATE1</italic>) under aluminum (Al<sup>3+</sup>) stress. However, the underlying mechanisms regulating <italic>VuMATE1</italic> expression remain unknown.</p> </list-item> <list-item> <p>We isolated and characterized a gene encoding Sensitive to Proton Rhizotoxicity1 (STOP1)‐like protein, <italic>VuSTOP1</italic>, from rice bean. The role of <italic>VuSTOP1</italic> in regulating <italic>VuMATE1</italic> expression was investigated using the yeast one‐hybrid assay. We characterized the function of VuSTOP1 in Al<sup>3</sup> <sup>+</sup> ‐ and H<sup>+</sup>‐tolerance using <italic>in planta</italic> complementation assays.</p> </list-item> <list-item> <p>We demonstrated that VuSTOP1 has transactivation potential. We found that <italic>VuSTOP1</italic> expression is inducible by Al<sup>3+</sup> and H<sup>+</sup> stress. However, although VuSTOP1 binds to the promoter of <italic>VuMATE1</italic>, the inconsistent tissue localization patterns of <italic>VuSTOP1</italic> and <italic>VuMATE1</italic> preclude VuSTOP1 as the major factor regulating <italic>VuMATE1</italic> expression. In addition, when a protein translation inhibitor increased expression of <italic>VuSTOP1</italic>, <italic> VuMATE1</italic> expression was inhibited. <italic>In planta</italic> complementation assay demonstrated that <italic>VuSTOP1</italic> could fully restore expression of genes involved in H<sup>+</sup> tolerance, but could only partially restore expression of <italic>AtMATE</italic>.</p> </list-item> <list-item> <p>We conclude that VuSTOP1 plays a major role in H<sup>+</sup> tolerance, but only a minor role in Al<sup>3+</sup> tolerance. The differential transcriptional regulation of <italic>VuSTOP1</italic> and <italic>VuMATE1</italic> reveals a complex regulatory system controlling <italic>VuMATE1</italic> expression.</p> </list-item> </list> </p> </abstract>